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TemBi 2014 mesocosm study: Summer storm impact on size-fractionated bacterial protein production and abundance of heterotrophic prokaryotes and picocyanobacteria

Description: We simulated an experimental summer storm in large-volume (~1200 m³, ~16m depth) enclosures in Lake Stechlin (https://www.lake-lab.de) by mixing deeper water masses from the meta- and hypolimnion into the mixed layer (epilimnion). The mixing included the disturbance of a deep chlorophyll maximum (DCM) which was present at the same time of the experiment in Lake Stechlin and situated in the metalimnion of each enclosure during filling. Size-fractionated Bacterial Protein Production (BPP) of particle associated (PA, >3.0 µm) and free-living bacteria (FL, 0.2-3.0 µm) (14C-Leu incorporation) as well as abundances of PA (microscopy of DAPI stained cells on 3.0 µm polycarbonate filters) and FL heterotrophic prokaryotes and picocyanobacteria (flow cytometry of SYBR green I stained cells) were monitored for 42 days after the experimental disturbance event. Mixing increased bacterial abundance and production about 3 weeks after mixing, which was associated to a mixing-induced stimulation of phytoplankton growth in the mixed enclosures compared to the controls. Simultaneously, decreased abundances of picocyanobacteria could be observed in mixed enclosures.

Global identifier:

Doi(
    "10.1594/PANGAEA.930921",
)

Types:
DataMeasurements(
    DataMeasurements {
        domain: Unspecified,
        station: None,
        measured_variables: [
            "Bacteria, free-living, abundance",
            "Bacterial abundance of HDNA-Bacteria",
            "Bacterial abundance of LDNA-Bacteria",
            "DATE/TIME",
            "DEPTH, water",
            "Day of experiment",
            "Day of the year",
            "Description",
            "Mesocosm label",
            "Nostocales biovolume",
            "Nostocales cell abundance",
            "Particle-associated bacterial abundance",
            "Picocyanobacteria abundance",
            "Protein production, free-living bacteria",
            "Protein production, particle associated bacteria",
            "Treatment",
        ],
        methods: [
            "14C-leucine incorporation",
            "Epifluorescence microscopy after DAPI staining",
            "Flow cytometry",
            "derived",
        ],
    },
)

Comment: Empty cells = NA

Tags: Polycarbonat ? Chlorophyll ? Filterbrunnen ? Seen ? Cyanobakterien ? Phytoplankton ? Bakterien ? Sturm ? Studie ? Klimawandel ? Wasseraufbereitung ? DCM ? bacterial production ? disturbance ? enclosure experiment ? mesocosm experiment ? summer storm ?

Region: Federal Republic of Germany

Bounding boxes: 13.0281° .. 13.0281° x 53.1433° .. 53.1433° 10.5° .. 10.5° x 51.5° .. 51.5°

License: Creative Commons Namensnennung 4.0

Language: Englisch/English

Organisations

Persons

Issued: 2021-04-29

Modified: 2025-08-22

Time ranges: 2014-08-04 - 2014-09-16

Resources

Status

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