Description: Stored surface seawater originally collected in the North Sea was amended with dissolved organic matter (DOM) obtained from the diatom Skeletonema marinoi by exposing the diatom culture to a hydrostatic pressure level of 40 MPa for 24 h. To this end, S. marinoi had been grown to stationary phase in L1 medium plus silicate (Guillard & Hargraves 1993) at 15°C under a light/dark regime of 14/10 h. Diatom-cell-free DOM was added to aliquoted seawater samples at initial concentrations of 250 µmol C/L. The microbial degradation of the added DOM and the microbial response to DOM-amendment at 15°C and in darkness was followed for 2 weeks. The seawater was subsampled at defined time intervals to analyze a number of variables.
Global identifier:
Doi(
"10.1594/PANGAEA.974879",
)
DataMeasurements(
DataMeasurements {
domain: Unspecified,
station: None,
measured_variables: [
"Bacteria",
"Carbon, organic, dissolved",
"Date/time end, experiment",
"Date/time start, experiment",
"Nitrogen, total dissolved",
"Polysaccharides",
"Proteins, total",
"Respiration rate, oxygen",
"Species, unique identification",
"Treatment: carbon",
"Treatment: duration",
"Type of study",
],
methods: [
"Fluorescence determination of DAPI stain",
"Lowry assay after Lowry et al. 1951",
"Oxygen logger, FireSting, Pyroscience",
"PAHBAH reducing sugar assay (Lever 1972)",
"Total organic carbon analyzer, Shimadzu, TOC-L",
],
},
)
Comment: Information about the used cultured organisms: Skeletonema marinoi, Bigelow National Center for Marine Algae and Microbiota, Strain No. CCMP1332, isolated from Long Island Sound (41.2264 N, -73.0639 W) (isolation date is unknown); https://ncma.bigelow.org/CCMP1332
Tags: Diatomeen ? Kohlenstoff ? Meerwasser ? Stickstoff ? Bakterien ? Studie ? Laborversuch ? Leckage ? Nordsee ? DOM ? DOM degradation ? culture experiment ? hydrostatic pressure ? microbial community ?
Bounding boxes: 7.841° .. 7.841° x 54.155° .. 54.155°
License: Creative Commons Namensnennung 4.0
Language: Englisch/English
Issued: 2025-08-03
Modified: 2025-08-03
Time ranges: 2019-04-07 - 2019-04-07
Accessed 1 times.