Description: Das Projekt "Genetische Wirkungen von Umweltchemikalien" wird vom Umweltbundesamt gefördert und von Universität-Gesamthochschule Essen, Klinikum durchgeführt. Objective: 1) Study of molecular interactions of environmental mutagens and carcinogens at DNA level. 2) to relate biological consequences of chemical mutagens to the extent and site of reaction between the mutagens and DNA. General information: 1) using (alkyl-deoxy nucleosis)-specific MAB in combination with appropriate immunoanalytical techniques, the molecular mechanisms of the interaction of alkylating carcinogens with genomic DNA of target cells, as well as the differential capacity of mammalian (human) cells to enzymatically detect and remove specific alkylation products in/from cellular DNA will be analyzed. 2) the spectrum of high affinity mab's will be expanded to include mab's specific for some of the reaction products of alkylating agents with DNA, in particular those used in the molecular dosimetry programme. Achievements: Monoclonal antibodies (MAB) specific for several alkylated deoxyribonucleic acid (DNA) components were characterised for sensitivity and cross reactivity. These MABs were found to be highly specific and suitable for detecting alkylated DNA components in human urine samples, even in the presence of a large excess of other purines. These MABs will enable the development of a rapid monitoring system for the detection and quantitation of 3-alkyladenine in DNA, ribonucleic acid (RNA) and body fluids of individuals previously exposed to alkylating agents.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Biopharmazeutikum ? Genom ? Kanzerogenität ? Säugetier ? Purin ? Alkylverbindung ? Biologische Wirkung ? DNA ? Dosimetrie ? Genetik ? Urin ? Kombinationswirkung ? Schadstoffwirkung ? Zytologie ? Urinprobe ? Antikörper ? Chemische Zusammensetzung ? Umweltchemikalien ? Krankenhaus ? Immunoassay ? Karzinogen ? Mutagener Stoff ? Zelle ? Studie ? Toxische Substanz ? Chemikalien ? Alkylierung ? MAB-Programm ? Mensch ? Monitoring ? Analyseverfahren ? Sensitivitätsanalyse ? Stofftrennung ? Umweltschutz ? Körperflüssigkeit ? RNA ? Enzymanalyse ? Monoklonale Antikörper ? Nachweisbarkeit ?
Region: Nordrhein-Westfalen
Bounding box: 6.76339° .. 6.76339° x 51.21895° .. 51.21895°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 1987-09-01 - 1991-08-31
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