Description: Das Projekt "Oxidative stress during degradation of phenolic compounds by bacterial ipso-hydoxylases - a challenge for the cell" wird vom Umweltbundesamt gefördert und von Eawag - Das Wasserforschungsinstitut des ETH-Bereichs durchgeführt. Preliminary results of transformation experiments with technical nonylphenol indicate that besides hydroquinone various alkylhydroquinones are produced as side products by ipso-hydroxylation. Because hydroquinones and alkylhydroquinones are agents of oxidative stress and have a high toxic potential, we plan to investigate the molecular processes associated with their formation and their further fate. In a metabolomics approach based on identification and quantification of putative metabolites by GC/MS and GC x GC/ToFMS, we intend to unravel the unique alkylhydroquinone fingerprint produced by ipso-degradation of technical nonylphenol in strain Bayram. There are indications that two reducing enzymes, a benzoquinone reductase that can quickly convert p-quinone into hydroquinone and a ferredoxin, able to reactivate TTNP3's hydroquinone-1,2-dioxygenase are involved in cell protection. We plan to elucidate the exact role of these enzymes in the metabolism of nonylphenols. The expected results will help us to shed new light on the metabolic strategies that microorganisms able to degrade potentially toxic phenolic substrates have evolved to cope with such substrates and their metabolites. This will be a major advance in the understanding of cellular responses to oxidative stress immanent to biodegradation of phenolic compounds.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Phenol ? Benzochinon ? Chinon ? Anfechtung ? Enzym ? Gaschromatografie ? Massenspektrometrie ? Nonylphenol ? Stoffwechselprodukt ? Stress ? Bakterien ? Biologischer Abbau ? Hydrolyse ? Nebenprodukt ? Oxidation ? Quantitative Analyse ? Reaktionsmechanismus ? Schadstoffabbau ? Toxische Substanz ? Zelle ? Mikroorganismen ? Endokrin aktive Substanz ? Schadstoffbildung ? Stoffwechsel ? Alkylhydrochinon ? Stoffwechselaktivität ? benzoquinone reductase ? ferredoxin ? hydroquinone ? hydroquinone-1,2-dioxygenase ? ipso-hydoxylase ?
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2010-05-01 - 2010-09-30
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