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Biological Regulation of Subsoil C-cycling under Field Conditions

Description: Das Projekt "Biological Regulation of Subsoil C-cycling under Field Conditions" wird vom Umweltbundesamt gefördert und von Universität Hohenheim, Institut für Bodenkunde und Standortslehre, Fachgebiet Bodenbiologie durchgeführt. The nature of the microbial communities inhabiting the deeper soil horizons is largely unknown. It is also not clear why subsurface microorganisms do not make faster use of organic compounds under field conditions. The answer could be provided by a reciprocal soil transfer experiment studying the response of transferred soils to fluctuations in microclimate, organic inputs, and soil biota. The subproject P9 will be responsible for the establishment of reciprocal transfer experiments offering a strong link between subgroups interested in organic matter quality, transport of organic substances, as well as functions of the soil microbial community. A single, high molecular weight substrate (13C labelled cellulose) will be applied at two different levels in the pre-experiment to understand the dose-dependent reaction of soil microorganisms in transferred surface and sub-soils. Uniformly 13C labelled beech roots - representing complex substrates - will be used for the main reciprocal soil transfer experiment. We hypothesize that transferring soil cores between subsoil and surface soil as well as addition of labelled cellulose or roots will allow us to evaluate the relative impact of surface/subsurface habitat conditions and resource availability on abundance, function, and diversity of the soil microbial community. The second objective of the subproject is to understand whether minerals buried within different soil compartments (topsoil vs. subsoil) in the field contribute to creation of hot spots of microbial abundance and activity within a period of two to five years. We hypothesize that soil microorganisms colonize organo-mineral complexes depending on their nutritional composition and substrate availability. The existence of micro-habitat specific microbial communities could be important for short term carbon storage (1 to 6 years). The third objective is to understand the biogeography and function of soil microorganisms in different subsoils. Parent material as well as mineral composition might control niche differentiation during soil development. Depending on size and interconnectedness of niches, colonization and survival of soil microbial communities might be different in soils derived from loess, sand, terra fusca, or sandstone. From the methodological point of view, our specific interest is to place community composition into context with soil microbial functions in subsoils. Our subgroup will be responsible for determining the abundance, diversity, und function of soil microorganisms (13C microbial biomass, 13C PLFA, enzyme activities, DNA extraction followed by quantitative PCR). Quantitative PCR will be used to estimate total abundances of bacteria, archaea and fungi as well as abundances of specific groups of bacteria at high taxonomic levels. We will apply taxa specific bacterial primers because classes or phyla might be differentiated into ecological categories on the basis of their life strategies.

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SupportProgram

Origin: /Bund/UBA/UFORDAT

Tags: Besiedlung ? Buche ? Biopharmazeutikum ? Schlichtemittel ? Substrat ? Bodenbiologie ? Zellulose ? DNA ? Humus ? Ökologie ? Skigebiet ? Brunnen ? Main ? Material ? Biogeografie ? Bodenkunde ? Bodenmikroorganismen ? Enzym ? Habitat ? Lössboden ? Regulierung ? Pflanzenwurzel ? Bakterien ? Sand ? Sandstein ? Organische Verbindung ? Oberboden ? Unterboden ? Mineralboden ? Bodenhorizont ? Untergrund ? Ackerland ? Bodenbildung ? Bodenfunktion ? Bodenorganismen ? Bohrkern ? Dosis ? Mikroorganismen ? Pilz ? Boden ? Stoff ? Wirkung ? Mikroklima ? Ressourcenverfügbarkeit ? Biomasse ? Mineral ? Fahrradfahren ? Verkehr ? Strategische Aspekte ? SICHT ? Eltern ? FUNKTION ? GEMISCHT ? ANGEWANDT ? DIFFERENZIERUNG ? GEWICHT ? Diversität ? GEWINNUNG ? GRUPPE ? KONTROLLE ? KURZ ? OBERFLAECHE ? WICHTIG ? Zins ? Betriebsvorschrift ? ABHAENGIG ? EINSATZ ? STARK ? ZEITRAEUME ? UEBERLEBEN ? UNTER ? CO2-Speicherung [CCS] ? DAUER ? VERANTWORTUNG ? VERBINDUNG ? BEREICH ? VERFRACHTUNG ? VERFUEGBARKEIT ? Bemessung ? EXPERIMENT ? VERHAELTNIS ? VERSCHIEBUNG ? SPEZIFISCH ?

Region: Baden-Württemberg

Bounding box: 9° .. 9° x 48.5° .. 48.5°

License: cc-by-nc-nd/4.0

Language: Deutsch

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Time ranges: 2013-01-01 - 2024-11-29

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