Description: Das Projekt "Molekulare Werkzeuge fuer die Bewertung des biologischen Sanierungspotentials an mit organischen Halogenen kontaminierten Standorten" wird vom Umweltbundesamt gefördert und von Gesellschaft für Biotechnologische Forschung mbH durchgeführt. Objective/Problems to be solved: Chlorinated hydrocarbons are the most important and widespread class of contaminants of soil and groundwater in all European countries and environmentally friendly methods have to be developed to abate this pollution. Many examples of bacterial transformation and, even, mineralisation of these compounds have been found. In field situations, stimulated or natural (intrinsic) bioremediation may, therefore, be a suitable remediation strategy for reducing risks. However, in practice, it is difficult to predict the bioremediation potential of the indigenous microbial population at polluted sites. Hence, there is a need for the development of effective, easy to handle tools for predicting degradative potential or for monitoring the effective stimulation of catabolic pathways in-situ. These tools should not be dependent on the culturability of contaminant-degrading organisms but, rather, be directed at the detection of the genes specific for microorganisms and genes encoding enzymes that catalyse the key reactions in the degradation pathways of contaminants. Scientific objectives and approach: Molecular detection methods will developed and optimised for the monitoring of bioremediation processes. A combination of detection of specific microorganisms, detection of catabolic genes and transformation activity, all in-situ, will allow the accurate analysis of in-situ natural attenuation. Results of the characterisation of intrinsic potentials of organohalogen-polluted sites by classical microcosm studies will be used to assess the usefulness of the molecular tools developed during the time-course of the project. A knowledge base created by the detailed genetic and biochemical characterisation of a collection of DNA segments encoding catabolic genes, will be used for the development of probes and primers for extracting the broadest possible diversity of genes from the environment. A database relating genetic structure to metabolic function for various key enzymes of halogenated hydrocarbon degradation will be built up and after optimisation of protocols for isolating DNA and RNA from environmental samples, as well as the adaptation of quantitative PCR methods, used for the development of specific probes and primers to analyse and quantify the presence and expression of degradative pathways in contaminated samples, to characterise enrichment cultures and to monitor the evolution of dechlorinating activity in microcosms. Results of this validation phase will be used to optimise the molecular tools already available. Finally, newly developed and optimised methods will be converted to an economic method to identify and quantify by molecular methods the natural attenuation potential of contaminated sites...
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Gen ? Chlorkohlenwasserstoff ? Altlast ? DNA ? Halogen ? Halogenkohlenwasserstoffe ? Bakterien ? Enzym ? Grundwasserverunreinigung ? Studie ? Evolution ? Bodensanierung ? Mineralisation ? Monitoring ? Politik ? Schadstoffabbau ? Standortwahl ? Mikroorganismen ? Biologische Behandlung ? Bodenschadstoff ? Bioremediation ? Soziale Aspekte ? Anreicherung ? in situ ? Risiko ? Schadstoffminderung ? Selbstreinigung ? Wirtschaftliche Aspekte ? Datenbank ? Sanierung ? enviromental protection ? renewable sources of energy ? scientific research ? legislation, regulations ? other energy topics ? RNA ?
Region: Lower Saxony
Bounding box: 9.16667° .. 9.16667° x 52.83333° .. 52.83333°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2000-01-21 - 2003-01-20
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