Description: Das Projekt "Ein analytisches System fuer die Messung von Mehrfachanalyten in Flusswasser" wird vom Umweltbundesamt gefördert und von Universität Tübingen, Institut für Physikalische und Theoretische Chemie, Abteilung Analytische Chemie durchgeführt. To achieve the objectives given above, the system envisaged requires a number of well integrated building blocks and methodologies: 1) Analyte specific elements will be antibodies raised against the analytes of choice. Polyclonal antibodies will be prepared according to established protocols. Specificity will be matched to the environmental problem addressed by appropriate immunisation protocols and/or blending of antibodies. The performance of immunochemistry will be assessed in a conventional ELISA tested. 2) For the detection of analytes a competitive test scheme will be implemented. ELISA results will serve as a reference. Competition will occur between the free analyte and compounds of the biochemical immobilisation systems conjugated with analyte derivatives. The detection will occur in a heterogeneous format, i e at the transducer surface. 3) Spatially resolved surface modification strategies (covalent and non-covalent) will be developed for the stable and reproducible patterned modification of the transducer surface to establish spatially resolved multi-reaction areas corresponding to the number of analytes. (Optical) surface analytical techniques and functional testing will be used to characterise surface chemistry. 4) An auxiliary immobilising system consisting of highly specific auxiliary bioaffinity compounds (antibodies or bioaffinity ligands) will serve to target the detection compounds to selected areas of the transducer surface. This novel idea is owned by GEC Marconi Ltd and is currently subject to patent applications (Ref. N 9216450.8/9216683.4/921743.4/9315995.2 in GB, 93917967.7 in Europe and 08-318,825 in the USA). 5) The transducer system will be based on fluorescence detection. A micro-optical/integrated optical transducer element will be used to allow effective excitation and spatially resolved detection of fluorophores bound to the surface. Mathematical modelling and systematic analysis of experimental results will be used to optimise the device. 6) The system setup will start with well defined interfaces (soft- and hardware) between compounds. Commercially available building blocks or units from commercially sold systems will be used where available. A system analysis will precede the implementation of an advanced demonstrator. 7) Validation will be carried out by two partners with a high degree of experience in analyzing complex environmental samples. The RIANA system will be tested in comparison with established reference techniques and procedures (instrumental analysis). Reference materials that were developed under the Measurement and Testing programme of the EU will be used. Where standardised methods do not exist neither at the national level nor at the European level US-EPA protocols will be adapted.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Tübingen ? Fluoreszenz ? Tierhaltungsanlage ? Fluss ? Flusswasser ? Altgerät ? USA ? Analytische Chemie ? Antikörper ? Chemische Analyse ? Gebäude ? Hardware ? Immunoassay ? Mischkunststoff ? Analyseverfahren ? Biochemische Methode ? Messverfahren ? Systemanalyse ? Systemtechnik ? Vergleichsanalyse ? Modellierung ? Europa ? Gewässer ? Immunsubstanz ?
Region: Baden-Württemberg
Bounding box: 9° .. 9° x 48.5° .. 48.5°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 1996-05-01 - 1999-04-30
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