Description: Das Projekt "The detection of uv b damage and characterisation of its biological consequences in the cellular components of human skin" wird vom Umweltbundesamt gefördert und von Universität Düsseldorf, Universitätshautklinik durchgeführt. Objective: The objective of this study is to obtain an understanding of the fundamental cellular mechanisms underlying the biological effects of human exposure of UV-B and sunlight. General Information: The project is a continuation of an existing highly successful collaboration (EV5V-CT91-0034) whose aim was to obtain a proper understanding of the fundamental mechanisms underlying the biological effects of human exposure to UV-B and sunlight. We will provide information on the activity of UV-B in the initiation step (via lethality, clastogenicity and mutability) and the promotional step (via immunomodulation and altered gene expression) of carcinogenesis. We will concentrate our studies on the in vitro response of cells derived from human skin to calibrated UV-B sources and natural sunlight. Subsequent studies will attempt to corroborate laboratory results with investigations on intact skin samples and ultimately on human volunteers. The experimental approach is based upon procedures which have proved successful in the present collaboration: (1) We will characterise the response of different cell types from human skin. (2) We will exploit our extensive resource of cells obtained from DNA repair defective donors. (3) We will utilise new and existing monoclonal antibodies to DNA damage to resolve the significance of specific lesions in studies of the lethality, mutagenicity and clastogenicity of UV-B. (4) We will resolve the contribution of UV-B to immunomodulation by a study of adhesion molecules and extend our study to altered gene expression. (5) We will provide methods to assess individual exposure. The programme of research is thus designed to provide a balance sheet of UV-B damage, its repair, and its effects on gene expression in human skin. From this balance sheet it may be possible to resolve the contribution will help to provide a scientific justification for well-focused protective or prophylactic measures and help to identify individuals at risk. Prime Contractor: MRC Cell Mutation Unit; Brighton; United Kingdom.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Düsseldorf ? Biopharmazeutikum ? Zellphysiologie ? Biologische Wirkung ? DNA ? Karzinogenese ? Mutagenität ? Solarstrahlung ? Strahlenwirkung ? Zytologie ? UV-Strahlung ? Vereinigtes Königreich ? Antikörper ? Strahlenexposition ? Bestrahlung ? Mensch ? Bewertungsverfahren ? Mortalität ? Bilanz ? Studie ? Vergleichsanalyse ? Wirkungsforschung ? Zelle ? Dermatose ? Organisches Gewebe ? Haut ? Reparatur ? in vitro ? Ressource ? Risiko ? Laboruntersuchung ? Adhäsion ? Versuchsperson ? clastogenicity ? immunomodulation ? mutability ? Genexpression ?
Region: Nordrhein-Westfalen
Bounding box: 6.76339° .. 6.76339° x 51.21895° .. 51.21895°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 1996-02-01 - 1999-01-31
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