Description: Das Projekt "Silica incorporation into newly synthesized cell walls and its effects on physiological properties of plant cells" wird vom Umweltbundesamt gefördert und von Universität Berlin (Humboldt-Univ.), Department für Nutzpflanzen- und Tierwissenschaften, Fachgebiet Phytomedizin durchgeführt. Silica increases the yield and mitigates biotic and a-biotic stresses in plants, but the mechanism of its action is only partially understood. Silica is thought to reduce the porosity of the cell wall and increase its stiffness, remove poisonous metals through co-deposition, and accelerate the response to fungal attacks. On the other hand, high silica content in leaves interferes with their digestion as fodder and fuel. The main goal of our research is to unravel the interactions of the plant cell with silica. We propose to study bio-mineralization in cultured plant cells and germinating pollen tissue at the molecular, ultrastructural, and micro/macro scale. We will use normal and surface-enhanced Raman microspectroscopy, X-ray scattering and electron microscopy, and multiphoton microscopy, respectively. The cell physiology state will be evaluated by molecular biology and bioinformatics tools. Specifically, we want to characterize the developing cell wall when cells are grown in solutions of high silicic acid concentrations, and to follow the time course of silicification. We will then evaluate the reaction of the cells to various stresses such as aluminum and heavy metal particles, high salt, high osmolarity, and viral pathogens. Based on our complementary views on silicification, we will illuminate pathways of silica-induced stress tolerance. This may allow developing plants that are drought resistant, or adapted to produce bio-fuel, or such that collect pollutants.
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Aluminiumoxid ? Aluminium ? Brennstoff ? Physiologische Wirkung ? Siliziumdioxid ? Berlin ? Biokybernetik ? Biologische Wirkung ? Osmose ? Schadstoffwirkung ? Schwermetall ? Salz ? Elektronenmikroskopie ? Zellwand ? Molekularbiologie ? Ökologische Potenz ? Belastungsfaktor ? Futtermittel ? Mikroskopie ? Stress ? Dürre ? Virusinfektion ? Metall ? Pilzbefall ? Salzgehalt ? Biologische Untersuchung ? Studie ? Vergleichsanalyse ? Organisches Gewebe ? Wirkungsanalyse ? Zelle ? Zellkultur ? Pflanze ? Beleuchtung ? Bioakkumulation ? Nanopartikel ? Keimung ? Krankheitserreger ? Partikel ? Pollen ? Zellphysiologie ? Inkorporation ? Pflanzenwachstum ? Porosität ? Bioinformatik ? Silifikation ? Wechselwirkung ? Kieselsäure ?
Region: Berlin
Bounding boxes: 13.41377° .. 13.41377° x 52.5233° .. 52.5233°
License: cc-by-nc-nd/4.0
Language: Englisch/English
Time ranges: 2012-05-01 - 2015-04-30
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