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Entgiftung toxischer Industrieabfaelle: Mikrobielle Degradation polychlorierter Biphenyle (PCBs) durch sequentielle anaerobe-aerobe Prozesse

Description: Das Projekt "Entgiftung toxischer Industrieabfaelle: Mikrobielle Degradation polychlorierter Biphenyle (PCBs) durch sequentielle anaerobe-aerobe Prozesse" wird vom Umweltbundesamt gefördert und von Bergische Universität-Gesamthochschule Wuppertal, Fachbereich 9 Naturwissenschaften II, Lehrstuhl für Chemische Mikrobiologie durchgeführt. Prior to the 1970s, PCBs were widely used for a variety of industrial purposes, including fluid-filled capacitors and transformers, hydraulic fluids, heat transfer fluids, plasticizers, and carbonless copy paper. The commercial mixtures used were complex mixtures of many homologs and isomers. 300,000 tonnes have entered the environment in widely disseminated form, 450,000 tonnes are either still in service or in landfills. Although storage in landfills has been used for PCBs in the past, their long environmental life has lead to the contamination of water, soil, air and even biological tissues. The proposed project consists of four parts: 1) Establishment of anaerobic microbial populations from polluted soil/sediments, capable of reductive dechlorination of highly chlorinated biphenyls to lower chlorinated biphenyls. Research is focused on the occurrence of this process in polluted soils and sediments, the kinetics of the process and the influence of various (environmental) factors, and the bacteria involved. 2) Establishment of aerobic bacterial cultures, capable of mineralizing low chlorinated biphenyls, by isolation from polluted soils/sediments or by in vivo-construction. Research is focused on the biochemistry and kinetics of degradation. 3) Genetic analysis of the isolated and in vivo-constructed aerobic PCB-degrading organisms and genetic engineering of these strains which includes i.e. the introduction of heavy metal resistance genes and marker genes. Research is focused on the molecular biology of PCB-degradation pathways and genes of heavy metal resistance. 4) Investigations on the biotechnology of the anaerobic/aerobic process. Research is focused on the procedure to clean contaminated soils and wastes in bioreactors. The goal of this project is to obtain information on: 1) the feasibility of a process to mineralize highly chlorinated biphenyls (formation of inorganic compounds like CO2, HCl) by a suitable sequential anaerobic-aerobic process and 2) its application (especially the factors which are determining the kinetics of the degradation process in a bioreactor).

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SupportProgram

Origin: /Bund/UBA/UFORDAT

Tags: Wuppertal ? Biphenyl ? Bleikontamination ? Hydraulikflüssigkeit ? Chlorwasserstoff ? Schwermetallresistenz ? Abfallbehandlung ? Aerobe Bedingungen ? Altpapier ? Anaerobe Bedingungen ? Polychlorierte Biphenyle ? Tracer ? Weichmacher ? Mikrobiologie ? Molekularbiologie ? Industrieabfall ? Kohlendioxid ? Biologische Abfallbehandlung ? Bodendegradation ? Anorganische Verbindung ? Bakterien ? Elektrischer Kondensator ? Dechlorierung ? Deponie ? Entgiftung ? Gentechnik ? Abfall ? Isomer ? Toxische Substanz ? Umweltverschmutzung ? Wasserverschmutzung ? Bodenluft ? Sediment ? Biotechnologie ? Organisches Gewebe ? Ökologischer Faktor ? Population ? Schadstoffelimination ?

Region: Nordrhein-Westfalen

Bounding box: 6.76339° .. 6.76339° x 51.21895° .. 51.21895°

License: cc-by-nc-nd/4.0

Language: Deutsch

Organisations

Time ranges: 1993-03-01 - 1996-08-31

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