Description: Das Projekt "Virusnachweis aus Oberflächengewässern" wird vom Umweltbundesamt gefördert und von Niedersächsisches Landesgesundheitsamt, Standort Aurich durchgeführt. Goals: A laboratory method for detection of enteropathogenic Viruses (e.g. Adenovirus) from surface (bathing) waters was established and five sampling sites monitored. The project aims at finding infectious routes in epidemiological cases. ; Approaches: A glasswool filtration column was used (similar to the method used in the EU-Virobathe project) to concentrate viruses from surface waters. The column was eluated by a pH-shift. The eluate was flockulated and virusparticles further concentrated by centifugation. Afterwards a Realtime-PCR was conducted for detection.; Results: A laboratory method for detection of Adenovirus in surface waters was established. The detection limit is around 10000 Virusparticles per 10 l. Recovery rates vary strongly. They seem to depend on suspended particles and other unknown factors. A mean recovery rate of 30 Prozent was achieved.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Aurich ? Glaswolle ? Schwebstoff ? Adenovirus ? Badegewässer ? Chemische Analyse ? Filter ? Epidemiologie ? Messverfahren ? Nachweisgrenze ? Oberflächengewässer ? pH-Wert ? Standortwahl ? Virus ? Wasserprobe ? Wasseranalyse ? Zentrifugation ? Verwertungsquote ? Krankheitserreger ? Probenahme ? Analytik ? Konzentrat ? Nachweisbarkeit ? PCR-Technik ?
Region: Lower Saxony
Bounding box: 9.16667° .. 9.16667° x 52.83333° .. 52.83333°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2006-09-01 - 2024-11-29
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