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GenOvotox - Development and Evaluation of a sensitive Replacement Method for Animal Experiments for the Detection of Endocrine Disrupting and Genotoxic Effects in Vertebrates

Description: Das Projekt "GenOvotox - Development and Evaluation of a sensitive Replacement Method for Animal Experiments for the Detection of Endocrine Disrupting and Genotoxic Effects in Vertebrates" wird vom Umweltbundesamt gefördert und von GenXPro GmbH durchgeführt. There is growing public concern about the increasing occurrence of manmade substances with hormone like activity - so called endocrine disrupting chemicals (EDCs). The identification of a given chemical as an EDC is routinely based on experiments with (sub-)adult mice or rats. The project GenOvotox aims to develop a replacement method for such tests using the chicken embryo. Both, responses on the structural (anatomical and histological alterations) and molecular level are used to identify specific effects of EDCs in very early embryonic development. These responses are evaluated for their suitability to be used in the future on a routine basis for the identification of EDCs in a regulatory context. The work programme of GenOvotox, a joint project between the companyGenXPro GmbH and our department, comprises the establishment, validation and commercialisation of a molecular screening tool by: - using SuperSAGE (Matsumura et al. 2003, 2005, 2006) for mRNA analysis and genome-wide quantification of gene activity in early developmental stages of the chicken embryo, - achieving a dose-effect relationship of endocrine disruptors on a structural and molecular level, - getting justifiable replacement, reduction and refinement of animal testing methods (Russel & Burch 1959) in the context of REACh.

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SupportProgram

Origin: /Bund/UBA/UFORDAT

Tags: Hirsch ? Huhn ? Bauprüfung ? Wirbeltier ? Embryo ? Endokriner Disruptor ? Dosis-Wirkungs-Beziehung ? Genotoxizität ? Maus ? Ratte ? Wirkung ? Erwachsener ? Embryonalentwicklung ? Analyseverfahren ? Bewertungsverfahren ? Chemikalien ? Dosis ? Hormon ? Prüfverfahren ? Tierversuch ? Wirkungsanalyse ? Tier ? Stoff ? Wirkung ? Endokrin aktive Substanz ? REACH ? Anthropogener Einfluss ? PROJEKT ? RATIFIKATION ? Bedenklicher Stoff ? ENTWICKLUNG ? ROUTINE ? ERSATZ ? EXPERIMENT ? SPEZIFISCH ? Siebung ? KOMMERZIALISIERUNG ? VERANTWORTUNG ? METHODE ? Vermehrung ? Werkzeug ?

Region: Hessen

Bounding box: 9° .. 9° x 50.55° .. 50.55°

License: cc-by-nc-nd/4.0

Language: Deutsch

Organisations

Time ranges: 2008-01-01 - 2011-12-31

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