Description: Das Projekt "Die Funktion einer Komponente des autonomen pathways bei der Blühzeitpunktkontrolle bei Arabidospis und Gerste" wird vom Umweltbundesamt gefördert und von Technische Universität Dresden, Lehrstuhl für Molekulare Zellphysiologie und Endokrinologie durchgeführt. We will compare the role of an RNA-binding protein in floral transition in Arabidopsis thaliana and Hordeum vulgare. The RNA-binding protein AtGRP7 promotes floral transition mainly by downregulating the floral repressor FLC via the autonomous pathway. Based on our observation that AtGRP7 affects the steady-state abundance of a suite of microRNA precursors, we will globally compare the small RNA component of the transcriptome during FTi regulation in wild type plants and AtGRP7 overexpressors by deep sequencing. This will extend the knowledge on small RNAs associated with floral transition and provide insights into the regulatory network downstream of this RNA-binding protein. Further, we will address the question how AtGRP7 orthologues function in crop species lacking FLC homologues. A barley line with highly elevated levels of the AtGRP7 orthologue HvGR-RBP1 shows accelerated FTi and preanthesis development when compared to a near-isogenic parent with very low expression of this gene. We will characterize in detail flowering of this line with respect to different photoperiods and its vernalization requirement. We will employ a TILLING approach to further delineate the function of HvGR-RBP1 in flowering. A candidate gene approach to identify downstream targets will provide insights into the signaling pathways through which HvGR-RBP1 influences FTi. This project contributes to the development of a functional cross-species network of FTi regulators, the major strategic aim of the SPP.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Gen ? Gerste ? Dresden ? Getreide ? Zellphysiologie ? Zierpflanze ? Vorläufersubstanz ? Blüte ? Endokrinologie ? Regulierung ? Pflanzenzüchtung ? Protein ? Globale Aspekte ? Änderung ? Staat ? PFAD ? BESTANDTEIL ? PROJEKT ? RNA ? STROMABWAERTS ? Sequenzierung ? VERGLEICH ? VERGLEICHEN ? BETRACHTUNG ? BEZUG ? Betriebsvorschrift ? ENTWICKLUNG ? ERFORDERNIS ? Eltern ? FRAGE ? FUNKTION ? GLEICHMAESSIG ? Genexpression ? KONTROLLE ? Kreuzung [biologisch] ? LAENDER ? NEU ? Art [Spezies] ?
Region: Sachsen
Bounding box: 10.40664° .. 10.40664° x 49.29433° .. 49.29433°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2011-01-01 - 2024-11-29
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