Description: Das Projekt "Untersuchungen zur Entwicklung und Validierung einer Testmethode zur Identifizierung endokriner Substanzen" wird vom Umweltbundesamt gefördert und von Fraunhofer-Institut für Molekularbiologie und Angewandte Oekologie durchgeführt. There is concern, that certain anthropogenic substances may modify the normal functioning of human and wildlife endocrine system. The endocrine system consists of glands and the hormones they produce guiding the development, growth, reproduction and behaviour of human beings and animals. Potential adverse effects of endocrine disrupters on humans and wildlife are controversially discussed among scientists, politicians and the public. Although there are associations between endocrine disrupting chemicals, so far investigated, and human health disturbances, a causative role of these chemicals in diseases and abnormalities possibly related to an endocrine disturbance has not been verified (CSTEE 1999). Concerning wildlife, there is strong evidence obtained from laboratory studies showing the potential of several environmental chemicals to cause endocrine disruption at environmentally realistic exposure levels. For investigating effects on wildlife, especially aquatic species, a number of bio-markers and screening tests are being developed, but so far, the ecological significance of the used endpoints for reproduction and population dynamics is not proven. This also applies for the relevance of environmental concentrations for compounds of concern. The aim of this project is to contribute to the development of testing strategies for endocrine disrupters. Due to the complexity of the endocrine system, the work focused on estrogen-receptor mediated processes being the mechanism of action of a large number of environmental chemicals. Within the scope of this project estrogen-receptor mediated reactions had been investigated at different levels from in-vitro systems up to the population level of fish (a) in intact cells, (b) in whole organisms, (c) on reproductive performance of a population and their usefulness for a tiered testing scheme had been discussed. With regard to the selection of in-vivo tests, it was not the intention to develop new tests but to proof the feasibility to enhance or refine, respectively, current test protocols, as agreed by several expert groups (OECD-EDTA, EPA-EDSTAC, CSTEE, EMSG). Deduced from these various levels of complexity following tests had been selected: 1. Transactivation assay in-vitro. Transactivation assays with recombinant receptor and recombinant reporter genes in yeast and mammalian cells were selected for testing the relative potencies of compounds to activate estrogen receptor mediated processes. 2. Assays towards the understanding of molecular mechanisms in intact cells. The expression of estrogen responsive genes, were investigated in a rat endometrium derived adenocarcinoma cell line (RUCA-I). These cells express relative high levels of estrogen receptor and exhibit estrogen inducible gene expression. usw.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Ethylendiamintetraessigsäure ? Populationsdynamik ? Fisch ? Endokrine Wirkung ? Gesundheitsschaden ? Raffination ? Tracer ? Molekularbiologie ? Endokriner Disruptor ? Bodendegradation ? Hefe ? OECD ? Ratte ? Umweltbelastung ? Umweltchemikalien ? Wirkung ? Geschlechtshormon ? Chemikaliensicherheit ? Fortpflanzung ? Endokrin aktive Substanz ? Bodenbewirtschaftung ? Bodenbelastung ? Bodenbildung ? Bodenfunktion ? Bodenorganismen ? Bodenuntersuchung ? Chemikalien ? Drüse ? Exposition ? Rechtsverfahren ? In-vivo-Test ? in vitro ? Mensch ? Prüfverfahren ? Störfall ? Studie ? Zelle ? Tier ? Stoff ? Hormonsystem ? Menschliche Gesundheit ? Wildlebende Tiere und Pflanzen ? Hormon ? Anthropogener Einfluss ? Krankheit ? Arbeit ? Sicherheitsanalyse ? Organismen ? System ? Potenzial ? Gesundheit ? Forschung ? Forschungseinrichtung ? Umwelt ? Strategische Aspekte ? Konzentrat ? Siebung ? öffentlich ? Auslese ? ökologisch ? Leistungsfähigkeit ? Mechanismen ? Bezug ? Umsetzbarkeit ? Methode ? Funktion ? On-Line-Betrieb ? Projekt ? Protokoll ? Verband ? Verhalten ? Ableitung ? Wachstum [biologisch] ? ausgewählt ? Gruppe ? In-Vivo ? Ausstellungsstück ? Ratifikation ? rekombinant ? Rezeptor ? stark ? Sachverständiger ? verwandt ? Screening [Voruntersuchung] ? Sieb ? Initiative ?
Region: Nordrhein-Westfalen
Bounding box: 6.76339° .. 6.76339° x 51.21895° .. 51.21895°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 1999-01-15 - 2000-07-01
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