Description: Das Projekt "Biotische und abiotische Mechanismen des Zurückhaltens und der Verbreitung der spongiformen Enzephalopathie im Boden" wird vom Umweltbundesamt gefördert und von Gesellschaft für Biotechnologische Forschung mbH durchgeführt. Scientific objectives: The interaction of the prion with both mineral and organic soil constituents, and its susceptibility to hydrolytic events mediated by soil microorganisms (free or associated with soil invertebrates), need to be studied, since very few information is presently available. Our measurable objectives are thus to have a better understanding of: 1. The mechanisms of adsorption of prions an soil mineral surfaces in order to know the most important factors affecting retention of prions in soils and possible modifications of conformation that may affect TSE infectivity. Expected achievements are a complete characterisation of the pH and ionic strength dependence of adsorption an clays, with a complete study of the structure of the adsorbed protein by FTIR, NMR and AFM techniques. 2. The mechanisms of interaction and entrapment of prions with soil organic matter as organic matter is also an important constituent of soil with properties markedly different from mineral constituents. Expected achievements are a characterisation of the differences in adsorption between clays and clay-organic complexes, as well as a comparison of immobilization efficiency by organic matter entrapment. 3. The mobility of prions in columns of soil in realistic conditions and with a comprehensive array of instrumentation and solution sampling equipment, with a study of the effect of water and redox potentials and of biotic factors such as the presence of soil microorganisms, of soil invertebrates or after sterilisation. Expected achievements are the constitution of a database containing prion migration data and the supporting data required from experimental soil columns for modelling. 4. The degradability of prions by the proteases of soil microorganisms which is an important question since infectious prion proteins are considered resistant to proteases, but the hydrolytic properties of soil microorganisms are usually able to degrade very recalcitrant molecules. Expected achievements are an evaluation of the proteolytic potential against prions of bacterial community from various soil microhabitats and detection of specific proteins following the contact with prions. 5. The Pate of prions after ingestion by soil invertebrates, such as earthworms, since, the probably enhanced dispersion of prions in soil resulting from their burrowing activities could be counterbalanced by the exposition of the prions, in their digestive tract, to proteolytic activity (microbial or gut secreted). Expected achievements are a data set describing accumulation of prion in the biomass of soil invertebrates, an identification of bacteria and Fungi playing the major role in prion proteolysis and a data set describing dynamics of prion in soil after passage through digestive tract of soil invertebrates. Prime Contractor: Institut National de la Recherche Agronomique Montpellier Montpellier. ...
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Regenwurm ? Ingestion ? Bodenbakterien ? Humus ? Schadstoffbelastung ? Adsorption ? Bodenmikroorganismen ? Bodenverunreinigung ? Wirbellose ? Organisches Material ? Wasserreinigung ? Lehm ? Mineralboden ? Biologischer Abbau ? Biochemische Methode ? Biotischer Faktor ? Bodenuntersuchung ? Daten ? Dispersion ? Epidemiologie ? Redoxpotential ? Schadstoffausbreitung ? Sterilisation ? Studie ? Tierkrankheit ? Wasseraufbereitung ? Pilz ? Biologische Aktivität ? Modellierung ? Effizienzvergleich ? Hygiene ? Risikofaktor ? Abbaubarkeit ? Krankheitserreger ? Mineral ? Persistenz ? Biomasse ? Datenbank ? Überlebensfähigkeit ?
Region: Lower Saxony
Bounding box: 9.16667° .. 9.16667° x 52.83333° .. 52.83333°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2003-01-01 - 2005-12-31
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