Description: Das Projekt "Spheroid-Based-Screen: Aufbau einer technologischen Plattform zum Einsatz eines 3D-Zellkulturmodells im industriellen Anti-Tumor-Wirkstoff-Screening-Prozess" wird vom Umweltbundesamt gefördert und von Universität Regensburg, Institut für Pathologie und Tumorimmunologie durchgeführt. The number of new drug candidates for the treatment of solid and metastatic malignancies identified and synthesized via modern high throughput technologies has consistently increased since only a few years. Driven by both ethical and economical incentives, this has already resulted in a shift of the test strategies used by the Pharmaceutical Industry in the early drug development process as reflected by the increased demand for and application of cell based assays. Currently available two-dimensional cell based assays, however, still reflect a highly artificial cellular environment. 3-D culture systems are known to better mimic the in vivo behavior of many cell types and are promising approaches for sophisticated in vitro drug screening. Scientific evidence from the past three decades indicates that these 3-D models can essentially contribute to a more efficient selection of the most promising drug candidates and the treatment modalities prior to and ultimately in place of animal testing (Kunz-Schughart et al., J. Biomol. Screen. 9:273, 2004). The objective of the project at the University of Regensburg (Institute of Pathology and Department of Pharmaceutical Technology) is to establish an advanced technological platform for the application of the spheroid 3-D culture system for high throughput screening of new anti tumor drug candidates. Our intention is to optimize, adapt and standardize this model system for industrial requirements. Cooperation with the industrial partner Avalon Pharmaceuticals Inc., MD, USA, shall guarantee a user oriented development of the platform. The evaluation of the culture system and platform includes the application of a defined number of well-known control substances/drugs to allow an adequate correlation of in vitro and in vivo data. A unique collection of standardized experimental protocols for the robust application of multiple human tumor cell lines in spheroid culture will build the base both to achieve general acceptance of the platform by the Pharmaceutical Industry and to conceptually provide the service of a spheroid culture and data archive located in Germany.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Pharmazeutische Industrie ? Arzneimittel ? Bundesrepublik Deutschland ? Gewährleistung ? Pathologie ? Gebäudetechnik ? Automatisierung ? Daten ? Tierversuch ? Tumor ? Wirkstoff ? Vermeidung von Tierversuchen ? Datenerhebung ? Dienstleistung ? Kulturtechnik ? MULTIPLE ?
Region: Bayern
Bounding box: 12.53381° .. 12.53381° x 47.795° .. 47.795°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2004-12-01 - 2007-02-28
Webseite zum Förderprojekt
https://www.tib.eu/de/filter?repno=0313143 (Webseite)Accessed 1 times.