Description: Das Projekt "Size-dependent effects of nanoparticles on the activity of cytochrome P450 isoenzymes" wird vom Umweltbundesamt gefördert und von ZMF - Center for Medical Research durchgeführt. Nanoparticles are known to be able to interfere with cellular metabolism and to cause cytotoxicity and moreover may interfere with specific cellular functions. Serious effects on the latter include changes in liver cell function. The cytochrome P450 system is expressed in many cells but is especially important in hepatocytes and hormone-producing cells. The interaction of polystyrene nanoparticles with the most important drug-metabolizing cytochrome P450 isoenzymes, CYP3A4, CYP2D6, CYP2C9 and CYP2A1 expressed individually in insect cells (BACULOSOMES ®) was studied by the cleavage of substrates coupled to a fluorescent dye. The data obtained for individual isoenzymes was compared to metabolism in microsomes isolated from normal liver and from the hepatoma cell line H4-II-E-C3.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Fluoreszenz ? Insekt ? Polystyrol ? Zellphysiologie ? Arzneimittel ? Biologische Wirkung ? Farbstoff ? Mikrosom ? Schadstoffwirkung ? Cytochrom ? Partikelgrößenverteilung ? Zytotoxizität ? Daten ? Hormon ? Leber ? Protein ? Wirkungsanalyse ? Wirkungsforschung ? Zelle ? Nanopartikel ? Stoffwechsel ? Cytochrom P450 ? Enzymaktivität ? Zellteilung ?
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2009-01-01 - 2009-08-31
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