Description: Das Projekt "Sichere Produktion eines Impfstoffs geegn Leishmaniose: Expression eines Leish-111 Antigens, lokalisiert im Plastom" wird vom Umweltbundesamt gefördert und von Universität für Bodenkultur Wien, Institut für Pflanzenbau und Pflanzenzüchtung durchgeführt. Production of pharmaceutics in plants is up to 50 times more cost effective than in fermenters through micro organisms. Plant derived drugs are an interesting alternative for developing countries where people cannot afford sufficient medical treatment. Therefore it makes sense to use this approach for synthesis of vaccines. Vaccines can be delivered as antigens either for peripheral or oral immunization. Transformation of plants with genes carrying selected antigens of the respective pathogen allows producing immunogenic proteins on the field with high yields. However, transformation of the nucleus leads to transgenic pollen which is spread to the environment. Our solution to this ecological problem consists in the alternative to transform the chloroplasts. In contrast to the nucleus tobacco plastids are not contained in the pollen, and thus can not be dispersed in the vicinity. Our goal is the production of an antigen vaccine against Leishmaniasis. According to the World Health Organization Leishmaniasis is one of the most serious, endemic parasitic infections afflicting the poor and disadvantaged in many countries of the world, particularly in North Africa, most of Asia, parts of the Middle East and much of South America. 12 million cases worldwide and an estimated 350 million people at risk for acquiring infection are assumed. In order to produce the antigenic Leish-111 epitope a chloroplast transformation vector will be constructed, which will contain a synthetic expression cassette for increased transcription conferred by two different promoters and synthetic ribosomal binding sites. Further the Leish-111 gene will be N-terminally fused to the sequence codons of a peptide which confers an increased translation efficiency. On transcriptional level this construct is followed by a selection cassette containing the aadA gene. Following selection on a medium containing spectinomycin positive transformants will be identified by PCR and Southern analysis. Further Western blot analysis will prove successful expression of the Leish-111 protein. Novel transformants are then tested on correct folding of the fusion protein. Depending on the upcoming results the next step will be to proof the immunogenicity and protectivity. The further work is aimed to set up an inducible antigen expression system, which is regulated through chemical induction.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Gen ? Chloroplasten ? Peptid ? Wien ? Weltgesundheitsorganisation ? Arzneimittel ? Lösungsmittel ? Zellkern ? Plastid ? Pflanzenproduktion ? Nordafrika ? Südamerika ? Leishmaniasen ? Naher Osten ? Endemie ? Medizin ? Pflanzenzüchtung ? Protein ? Standortwahl ? Synthese ? Tabak ? Amerika ? Asien ? Biotechnologie ? Chemikalien ? Entwicklungsland ? Mittlerer Osten ? Körperschaft ? Krankheitserreger ? Pollen ? Risiko ? Industrieland ? Infektion ? Blotting ? PCR-Technik ? Antigen ? Promotor [Gen] ? Sequenzierung ? Transkription ? Dispergierung ? Impfstoff ? Impfung [Medizin] ?
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2008-02-01 - 2011-12-31
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