Description: Das Projekt "Struktur-Funktionsbeziehungen bei Eisenschwefelproteinen" wird vom Umweltbundesamt gefördert und von Gesellschaft für Biotechnologische Forschung mbH durchgeführt. Iron-sulfur clusters are ubiquitously present in nature; they function in redox catalysis-hydrolytic catalysis-synthesis of DNA-precursors, regulation of messenger-RNA transcription, and secondary electron transfer. These clusters are essential for respiration, photosynthesis, intermediary metabolism, metal homeostasis. Redox transitions are pivotal in the biological functioning of iron-sulfur clusters: They are directly related to catalytic activity, to activation-deactivation phenomena, to affinity for STEM-LOOP structures in RNA, and to electron-transfer rates. The relationships of redox properties with cluster structures and protein surroundings are not well understood. The present proposal aims at combining the strength of different laboratories, specialized in the field and with different experiences, to undertake a concerted effort to determine these relations. It is proposed to exploit the complementarity of several advanced physio-chemical techniques, notably, Mossbauer, EPR, ENDOR, and Paramagnetic NMR to obtain information on the spin states of the individual irons in the clusters, and hence on the electronic distribution at low and high temperatures. This key information is related to the redox potentials of the individual irons in the cluster, which can then be compared with the overall redox potentials obtained from electrochemical methods. Structural data is available for certain systems, and will be determined for other systems in X-ray crystallographic, molecular modelling and NMR studies. It is the first time that such an approach is systematically applied to metal clusters in biological systems. The proposers are able to isolate and purify iron-sulfur proteins of many different types from different sources recombinant proteins can also be produced. This will permit the planning and expression of site-directed mutants of fine-tune redox and magnetic properties, and thus to test hypothesis of structure-function relationships coming out from the analysis of physico-chemical measurements. The expected objective is to establish general relations between the (MACROSCOPIC) redox properties and the (MICROSCOPIC) electronic and magnetic structure as a function of molecular structure for the different classes of biological iron-sulfur clusters. Thus will lay the foundation for a detailed understanding of the functioning of iron-sulfur clusters in many vital processes on a molecular and atomic level.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Biopharmazeutikum ? Mutation ? Röntgenstrahlung ? Eisen ? Elektrochemie ? Temperaturabhängigkeit ? Temperaturverteilung ? Photosynthese ? Spektralanalyse ? Metall ? Proteinbiosynthese ? Elektrochemisches Verfahren ? Atmung ? Daten ? Gewerbebetrieb ? Protein ? Redoxpotential ? Studie ? Systemanalyse ? Modellierung ? Kenngröße ? Chemische Reaktion ? Stoffwechsel ? Informationsgewinnung ? Chemisch-physikalische Behandlung ? ENDOR ? Elektronen ? Molekülstruktur ? Mossbauer ? Cluster-Struktur ? Paramagnetic-NMR ? Röntgenstrukturanalyse ? EPR ? Eisen-Schwefel-Proteine ?
Region: Lower Saxony
Bounding box: 9.16667° .. 9.16667° x 52.83333° .. 52.83333°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 1994-11-01 - 1997-10-31
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