Description: Das Projekt "Functional analysis of genes and enzymes involved in biofilm formation - Structural modifications of the expolysaccharide PIA and peptidoglycan in Staphylococcus" wird vom Umweltbundesamt gefördert und von Universität Tübingen, Institut für Mikrobielle Genetik durchgeführt. In numerous reports of the past two decades, it has been shown that especially biofilmforming staphylococci cause a new infection that is best described as 'chronic polymer-associated infection'. By genetic analysis we could show that two cell wall structures, the Polysaccharide Intercellular Adhesin PIA, alpha beta-1.6-linked N-acetylglucoseamine polymer, and the peptidoglycan, play a rote in biofilm formation. PIA is partially de-acetylated and we want to find out which gene and enzyme is responsibte for this reaction and how deacetylation alteres the physico-chemical properties of PIA, the bacterial growth and biofilm formation, and the biological activity of PIA especially immune modulation. IcaB is the most likely candidate for PIA de-acetylation, however, the final enzymatic prove is still lacking. In a search for biofilm-negative mutants we identified a new gene which we named tentatively oatA because it shows similarity to peptidoglycan 0-acetylases. The corresponding deletion mutant lacks biofilm formation an polystyrene or glass surface and is also sensitive to lysozyme, an important defense enzyme of the innate immune system. We plan to compare the peptidoglycan structure of wild-type and AoatA by HPLC/MS-analysis and to purify and characterize the OatA enzyme. The two topics deal with the modulation of two different cell wall structures, namely PIA and peptidoglycan. Although the structures are different, the studied genes, icaB and oatA, share some common features: They are both important for biofilm formation and the corresponding enzymes may catalyze related reactions de-acetylation (IcaB) and acetylation (OatA).
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Tübingen ? Gen ? Polystyrol ? Lack ? Arsen ? Genetik ? Polymer ? Staphylokokken ? Wild ? Zellwand ? Biofilm ? Enzym ? Flüssigkeitschromatografie ? Immunsystem ? Biologische Aktivität ? Infektion ? Deletion ?
Region: Baden-Württemberg
Bounding box: 9° .. 9° x 48.5° .. 48.5°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2001-07-01 - 2006-05-31
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