Description: Das Projekt "Mechanisms regulating the boron nutritional status in rapeseed and Arabidopsis and their implications for the development of boron-efficient genotypes" wird vom Umweltbundesamt gefördert und von Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung durchgeführt. Boron (B) is an essential microelement for plants. Despite the use of modern fertilization methods, B deficiency still causes losses in agricultural plant production. Even though many positive effects of B on plant growth and physiology have been reported, a large majority of B functions and the regulatory mechanisms controlling the B nutritional status remain unknown. The main objective of this project is to elucidate how the greatly B deficiency-sensitive Brassica crop plants process and regulate their B status during vegetative and reproductive growth. In this context, the project aims at identifying the mode of action of B in mechanisms regulating the B status itself and uncovering those mechanisms contributing to B efficiency in different genotypes. Plant species subjected to investigation will be the agronomically important oilseed and vegetable plant Brassica napus (rapeseed) and its close relative the genetic and molecular model plant Arabidopsis thaliana. Questions addressed within the scope of this project should lead to a detailed understanding of mechanisms controlling B uptake and allocation from the level of the whole plant down to the cellular level. B transport routes and rates will be determined in sink- and source tissues and in developmental periods with a particularly high B demand. A special focus will be on the identification of B transport bottlenecks and the analysis of B deficiency-sensitive transport processes to and within the highly B-demanding reproductive organs. Recent studies in Arabidopsis suggest that Nodulin26-like Intrinsic Proteins (NIPs), which belong to the aquaporin channel protein family, are essential for plant B uptake and distribution. The systematic focus on the molecular and physiological characterization of B. napus NIPs will clarify their role in B transport and will identify novel NIP-associated mechanisms playing key roles in the B response network.To further resolve the mostly unknown impact of the B nutritional status on gene regulation and metabolism, a transcript and metabolite profile of B-sufficient and B-deficient rapeseed plants will be generated. Additionally, an Arabidopsis transcription factor knockout collection (greater 300 lines) will be screened for abnormalities in responses to the B nutritional status. This will identify yet unknown B-responsive genes (transcription factors and their targets) and gene products (enzymes or metabolite variations) playing key roles in signalling pathways and mechanisms regulating the B homeostasis. Boron (in form of boric acid) and arsenite (As) share in all likelihood the same NIP-mediated transport pathways. To assess the consequences of this dual transport pathway the so far unstudied impact of the plants B nutritional status on the accumulation and distribution of As will be investigated in B. napus. Moreover, the current dimension of the As contamination of Brassica-based food products, to which consumers are exposed to, will be analyzed. usw.
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Gen ? Ölsaat ? Gemüse ? Getreide ? Raps ? Wasserstraße ? Befruchtung ? Bor ? Borsäure ? Genetik ? Kulturpflanze ? Verkehrsweg ? Blei ? Stoffwechselprodukt ? Enzym ? Pflanzenart ? Pflanzenproduktion ? Wirkung ? Familie ? Fortpflanzung ? Rechtsverfahren ? Organisches Gewebe ? Verbraucherprodukt ? Kontrollmaßnahme ? Pflanzenernährung ? Protein ? Studie ? Wirkungsgrad ? Pflanze ? Schaden ? Wirkung ? Produktion ? Anreicherung ? Lebensmittel ? Organ ? Produkt ? Stoffwechsel ? Verkehr ? Änderung ? Verunreinigung ? ENTWICKLUNG ? METHODE ? Art [Spezies] ? Siebung ? Transkription ? UNTERSUCHUNG ? FORMBLAETTER ? VERANTWORTUNG ? VERARBEITEN ? VERFRACHTUNG ? VERTEILUNG ? WICHTIG ? Wachstum [biologisch] ? ZEITRAEUME ? FRAGE ? NACHFRAGE ? ABHOLEN ? BESCHREIBUNG ? FUNKTION ? Fahrwegfestlegung ? BESONDER ? BEWERTEN ? Genregulation ? NEU ? PFAD ? PROJEKT ? Bemessung ? Bepflanzung ? DAUER ? MECHANISMEN ? STILLGELEGT ?
Region: Sachsen-Anhalt
License: cc-by-nc-nd/4.0
Language: Englisch/English
Time ranges: 2012-01-01 - 2025-03-31
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