Description: Das Projekt "Teilprojekt: IPHT Jena" wird vom Umweltbundesamt gefördert und von Leibniz-Institut für Photonische Technologien e.V. durchgeführt. Globally, nearly 6,000 children die each day due to water-related illnesses. Treatment based approaches must be implemented to minimize these deaths. Rapid (less than 1 hr) detection platforms covering most waterborne pathogens of concern, their indicators, and associated sources of antibiotic resistance bacteria on a single chip are urgently needed. Such platforms must be operable under field conditions with personnel requiring minimal training. This proposal focuses on such a multiplexed chip by adapting an already developed robust and low cost platform (Gene-Z) for on-site water pathogen detection. Genetic markers associated with at least a dozen waterborne pathogens, indicators, and antibiotic resistance bacteria are included on the chip including viability testing to be validated with appropriate sensitivity and specificity. The proposed project has three objectives: 1) Provision of waterborne pathogens chips and detection systems, 2) Integration of Live vs. Dead (Viability) Protocol on the Chip, and 3) Field Validation, Deployment, Support and Feedback. When fully developed and validated, the chip and platform will provide the a number of key benefits compared to other existing technologies and approaches including fast results, ease of use, specificity, sensitivity, and low cost. Differentiating characteristic compared to other molecular biology technologies include multiplexing of bacteria and protozoan, use of multiple virulence markers, live vs. dead differentiation, and measurement of antibiotic resistance genes. The consortium combines academic and industry partners with expertise in molecular biology, bioanalytics, and on-site detection technology development.
Types:
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Jena ? Gen ? Antibiotikaresistenz ? Biomarker ? Genetik ? Sensor ? Tracer ? Molekularbiologie ? Kind ? Kosten-Nutzen-Analyse ? On-Site-Verfahren ? Protozoen ? Verkeimung ? Bakterien ? Analyseverfahren ? Niedrigwasser ? Schnelltest ? Pathogenität ? Wasseranalyse ? Wasserverschmutzung ? Biologische Kontamination ? Gutachten ? Krankheitserreger ? Keim ? Lebend-Tot-Differenzierung ? Lebendkeim ? Validierung ?
Region: Thuringia
Bounding box: 11° .. 11° x 50.91667° .. 50.91667°
License: cc-by-nc-nd/4.0
Language: Deutsch
Time ranges: 2016-07-01 - 2019-06-30
Webseite zum Förderprojekt
https://www.tib.eu/de/filter/?repno=01DQ16009A (Webseite)Accessed 1 times.