Description: Das Projekt "Can community transcription profiles be used to predict environmental biotransformation of organic contaminants?" wird vom Umweltbundesamt gefördert und von Eidgenössische Anstalt für Wasserversorgung, Abwasserreinigung und Gewässerschutz durchgeführt. Rapid introduction of new synthetic chemicals into commerce as well as the tens of thousands of legacy chemicals with no appropriate environmental fate information have created the need for tools that predict the fate of these chemicals in the environment. Biodegradation is a primary mechanism potentially reducing the risk of exposure to these chemicals, but incomplete biodegradation may result in an accumulation of biotransformation products that may have similar detrimental effects as their parent chemicals to both human and ecosystem health. Structure-biodegradation relationships have been developed as tools to predict biotransformation half-lives and pathways, but lack specificity for individual environmental compartments, leading to large uncertainties in the prediction of half-lives and over-prediction of biotransformation products that may be formed. In this proposal, we propose to investigate the possibility to improve the specificity of biotransformation prediction by considering not only chemical structure, but also the metabolic potential of the microbial communities residing in the environment in which the compound may undergo biotransformation. The aim of this work is to test the hypothesis that gene transcription levels in microbial communities can be used to predict the extent of specific biotransformation reactions. We will do this by simultaneous characterization of microbial communities in terms of their biotransformation capacity and their gene transcription profile using metatranscriptome sequencing. We will test the hypothesis at the level of well-established enzyme-compound pairs to investigate the utility of the metatranscriptomic approach in an environmental system setting. The results of this inherently interdisciplinary project are expected to significantly contribute to the fields of environmental chemistry and environmental microbiology. Most directly, the results will facilitate more accurate and community-specific predictions of biotransformation of synthetic organic chemicals. Further, we envision contributions to improving accuracy in environmental fate and persistence modeling, development of bioremediation/bioaugmentation strategies, and shedding light on fundamental questions about microbial community formation and structure and how that relates to community function.
SupportProgram
Origin: /Bund/UBA/UFORDAT
Tags: Gen ? Umweltchemie ? Genetik ? Kläranlage ? Mikrobiologie ? Biologische Abbaubarkeit ? Bodenmikroorganismen ? Chemische Analyse ? Enzym ? Katalyse ? Software ? Umweltbelastung ? Umweltchemikalien ? Prognose ? Organische Verbindung ? Interdisziplinarität ? Abiotischer Abbau ? Halbwertszeit ? Biologischer Abbau ? Altlastensanierung ? Prognosemodell ? Risikoanalyse ? Organischer Schadstoff ? Schadstoffabbau ? Stoffbewertung ? Biotransformation ? Umweltplanung ? Chemikalien ? Mikroorganismen ? Sediment ? Modellierung ? Abbau ? Ökosystem ? Persistenz ? Reaktionskinetik ? Risiko ? Standortbedingung ? Schadstoff ? Chemische Reaktion ? Modellrechnung ? Biodegradability prediction ? Analytik ? Biotransformation pathways ? Genexpression ? Grundlagenforschung ? Microbial community ?
License: cc-by-nc-nd/4.0
Language: Englisch/English
Time ranges: 2013-01-01 - 2015-12-31
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