Description: Schettgen, Thomas; Bertram, Jens; Kraus, Thomas; Kolossa-Gehring, Marike Analytical Methods 13 (2021), 1847-1856; online 16. März 2021 Methylisothiazolinone and the reaction mixture of chloromethylisothiazolinone/methylisothiazolinone (MCI/MI, 3 : 1) are broadly used biocides that are contained in many products of everyday life (e.g. cosmetics, wet wipes, etc.). As MI and MCI are able to sensitize (and penetrate) the skin, their application in cosmetic products is of concern. In previous work, we have revealed a background exposure of the general population to MI and/or MCI/MI (3 : 1) by the determination of urinary N-methylmalonamic acid (NMMA) as the main human metabolite. To corroborate these findings, we have now developed a two-dimensional LC/MS/MS method for the quantification of a mercapturic acid metabolite of MI and MCI ((acetylamino){[3-(methylamino)-1-(methylthio)-3-oxopropyl]thio}acetic acid or shortly “M-12”) in human urine. This analyte is enriched online using a Strata-X-column and stripped from the urinary matrix. Then, the analyte is back flushed to the analytical column (Phenomenex C18(2), 150 × 4.6 mm) and finally quantified by tandem mass spectrometry with the use of isotopically labelled M-12 as the internal standard. The LOQ for M-12 was 0.2 ng mL−1 urine and sufficient to quantify urinary background levels. Precision within and between series for M-12 in urine at concentrations varying from 0.2 to 5 ng mL−1 ranged from 2.1 to 23.9% and accuracy ranged from 86.3 to 101.8%. Mean accuracy for M-12 in individual urine samples was 94.3% (range: 89.7–102.9%). We applied this method to previously collected 24 h urine samples of 60 persons with no specific exposure to MI and/or MCI/MI (3 : 1). The metabolite M-12 could be quantified in each urine sample. The median and 95th percentile levels for urinary M-12 were determined to be 0.62 and 2.26 ng mL−1, respectively. In these urine samples, the concentrations of the previously determined metabolite NMMA and M-12 correlated well. In the future, we will apply this method to urine samples of a previously conducted human exposure study to explore the additional value of M-12 as a biomarker of exposure to MI and MCI. doi: 10.1039/d1ay00183c
Global identifier:
Doi(
"10.1039/D1AY00183C",
)
Types:
Text {
text_type: Publication,
}
Origin: /Bund/UBA/Umweltprobenbank
Tags: Biozid ? Biomarker ? Urin ? Main ? Urinprobe ? Massenspektrometrie ? Stoffwechselprodukt ? Analyseverfahren ? Haut ? Kosmetisches Mittel ? Studie ? Hintergrundwert ?
License: other-closed
Language: Deutsch
Issued: 2021-01-01
Time ranges: 2021-01-01 - 2021-01-01
doi: 10.1039/d1ay00183c
https://doi.org/10.1039/D1AY00183C (Webseite)Accessed 1 times.