Das Projekt "The response of tree root systems to interacting above ground stress" wird vom Umweltbundesamt gefördert und von Eidgenössische Forschungsanstalt für Wald, Schnee und Landschaft durchgeführt. The proposed project aims to identify carbohydrate-related genes in poplar roots that are regulated by above ground stress. Ozone, elevated CO2 defoliation by clipping and shading will be applied to poplar in order to modulate the carbohydrate metabolism. Stress regulated genes will be identified by application of oligonucleotide microarray analyses and the expression of carbohydrate-related genes will be quantified. Gene expression profiling will be complemented with biochemical analysis of metabolites and carbohydrate-metabolising enzymes in poplar roots.