Das Projekt "Protocol for the evaluation of residues in industrial contaminated liquid effluents" wird vom Umweltbundesamt gefördert und von Fraunhofer-Institut für Toxikologie und Aerosolforschung durchgeführt. Objective: To perform the chemical analysis of compounds which are present in industrial effluents and important for their persistency, bio accumulative potential, toxicological and ecotoxicological activity. General Information: The project includes a global analysis on the samples followed by fractionation of the organic compounds into groups of different polarity by extraction at different pH's and with different materials and by further fractionation by HPLC, using for the analysis a series of powerful techniques, including LC-MS, GC-MS and NMR; a simple and cost-effective ecotoxicological characterisation of the samples and their fractions, using the most appropriate of a series of rapid screening tests, such as Microtox and the Daphnia Inhibitory Quotient (IQ) will be made. The project consists of three main tasks: -A multiple ecotoxicological approach for industrial effluents hazard evaluation. -Characterisation of the chemical composition of the effluent, and integration with the data from the first task. -Validation of the developed protocol. To develop and assess this general protocol and the individual elements of the approach, at least three authentic situations will be considered and liquid effluents of industrial origin will be studied. In particular, industries with different production and leachate from industrial wastes will be considered. Prime Contractor: Instituto di Richerche Farmacologiche Mario Negri; Milano; Italy.