Flowering time (FTi) genes play a key role as regulators of complex gene expression networks, and the influence of these networks on other complex systems means that FTi gene expression triggers a cascade of regulatory effects with a broad global effect on plant development. Hence, allelic and expression differences in FTi genes can play a central role in phenotypic variation throughput the plant lifecycle. A prime example for this is found in Brassica napus, a phenotypically and genetically diverse species with enormous variation in vernalisation requirement and flowering traits. The species includes oilseed rape (canola), one of the most important oilseed crops worldwide. Previously we have identified QTL clusters related to plant development, seed yield and heterosis in winter oilseed rape that seem to be conserved in diverse genetic backgrounds. We suspect that these QTL are controlled by global regulatory genes that influence numerous traits at different developmental stages. Interestingly, many of the QTL clusters for yield and biomass heterosis appear to correspond to the positions of meta-QTL for FTi in spring-type and/or winter-type B. napus. Based on the hypothesis that diversity in FTi genes has a key influence on plant development and yield, the aim of this study is a detailed analysis of DNA sequence variation in regulatory FTi genes in B. napus, combined with an investigation of associations between FTi gene haplotypes, developmental traits, yield components and seed yield.
Goal of the project EcoTopTen 2.0 is increasing the supply and demand for highly energy-efficient ecological products and to motivate consumers through targeted Actions, to reduce greenhouse gas emissions and to relieve the environment. Importantelements are the regular update of EcoTopTen market overviews, cooperations with retailers, the action 'Save 1000 kWh', as well as accompanying social media activities.
In many plant species, FLOWERING LOCUS T and related proteins are the mobile signal that communicates information on photoperiod from the leaves to the shoots, where the transition to flowering is realized. FT expression is tightly controlled at the transcriptional level so that it is restricted to leaves, occurs only in appropriate photoperiods, and integrates ambient temperature and developmental cues, as well as information on biotic and abiotic stress. We previously established that FT transcription in the model plant Arabidopsis thaliana requires proximal promoter cis-elements and a distal enhancer, both evolutionary conserved among Brassicacea species. In addition, FT transcription is blocked prior vernalization in biannual accessions and vernalization-dependency of FT is controlled through a CArG-box located in the first intron that binds the transcriptional repressor FLOWERING LOCUS C (FLC). Chromatin-mediated repression by the Polycomb Group (PcG) pathway is required for photoperiod-dependent FT regulation and participates in FT expression level modulation in response to other cues.In this project, I propose to explore the available sequence data from the 1001 genome project in Arabidopsis to evaluate how often changes in regulatory cis-elements at FT have occurred and how these translate into an adaptive value. Allele-specific FT expression pattern will be measured in F1 hybrids of different accessions in response to varying environmental conditions. FT alleles that show cis-regulatory variation will be further analyzed to pinpoint the causal regulatory changes and study their effect in more detail. The allotetrapolyploid species Brassica napus is a hybrid of two Brassiceae species belonging to the A- and C-type genome, which are in turn mesopolyploid due to a genome triplication that occurred ca. 10x106 years ago. We will determine allele-specific expression of FT paralogs from both genomes of a collection of B. napus accessions. The plants will be grown in the field in changing environmental conditions to maximize the chance to detect expression variation of the paralogs. We will compare the contribution of the founder genomes to the regulation of flowering time and asses variation in this contribution. A particular focus will be to study the impact of chromatin-mediated repression on allele selection in B. napus.
An 7 Messtellen in der Schweiz werden waehrend der Vegetationsperiode die Inhalte von 1 m an Pollen und Pilzsporen gemessen. Die Messtellen sind in chronologischer Reihenfolge aufgefuehrt: Basel, seit 1969, Davos seit 1972, Genf seit 1978 (bezahlt von Prof. Dr.med. G.Boehm), Neuenburg seit 1979 (?), Zuerich seit 1981, Lugano seit 1983 und Samedan seit 1983. Fuer Neuchatel und Lugano hat die Projektleiterin Biologen ausgebildet. Diese teilen woechentlich die Resultate der letzten beiden untersuchten Tage mit. Die uebrigen Messtellen werden in Basel ausgewertet. Dafuer muss woechentlich die 'Trommel' der Burkard-Pollen- und Sporenfalle hin- und hergeschickt werden. Die Informationen werden monatlich den Aerzten, woechentlich Zeitungen, Radio, Nr.162 des Telefons mitgeteilt als Hilfe fuer Heufieberpatienten. Auch die Pilzsporen der Luft werden erfasst. (Ca. 20 versch. Arten.) Dabei werden auch Emissionen von Industrie und Verbrennungsanlagen untersucht. Prof. Boehm untersucht diese in einem Extra-Projekt. Der Informationsdienst soll Heuschnupfenpatienten orientieren.