Das Projekt "The effect of elevated atmospheric CO2 concentration on gross nitrogen dynamics, plant N-uptake and microbial community dynamics in a permanent grassland" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Gießen, Institut für Pflanzenökologie (Botanik II).To predict ecosystem reactions to elevated atmospheric CO2 (eCO2) it is essential to understandthe interactions between plant carbon input, microbial community composition and activity and associated nutrient dynamics. Long-term observations (greater than 13 years) within the Giessen Free Air Carbon dioxide Enrichment (Giessen FACE) study on permanent grassland showed next to an enhanced biomass production an unexpected strong positive feedback effect on ecosystem respiration and nitrous oxide (N2O) production. The overall goal of this study is to understand the long-term effects of eCO2 and carbon input on microbial community composition and activity as well as the associated nitrogen dynamics, N2O production and plant N uptake in the Giessen FACE study on permanent grassland. A combination of 13CO2 pulse labelling with 15N tracing of 15NH4+ and 15NO3- will be carried out in situ. Different fractions of soil organic matter (recalcitrant, labile SOM) and the various mineral N pools in the soil (NH4+, NO3-, NO2-), gross N transformation rates, pool size dependent N2O and N2 emissions as well as N species dependent plant N uptake rates and the origin of the CO2 respiration will be quantified. Microbial analyses will include exploring changes in the composition of microbial communities involved in the turnover of NH4+, NO3-, N2O and N2, i.e. ammonia oxidizing, denitrifying, and microbial communities involved in dissimilatory nitrate reduction to ammonia (DNRA). Stable Isotope Probing (SIP) and mRNA based analyses will be employed to comparably evaluate the long-term effects of eCO2 on the structure and abundance of these communities, while transcripts of these genes will be used to target the fractions of the communities which actively contribute to N transformations.
Das Projekt "Forschergruppe (FOR) 1525: INUIT - Ice Nuclei research UnIT, In-situ Messungen von eiskeimbildenden Partikeln (INP) und quantitative Bestimmung von biologischen INP" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Johann Wolfgang Goethe-Universität Frankfurt am Main, Institut für Atmosphäre und Umwelt.Die Bildung der Eis Phase in der Troposphäre stellt einen wichtigen Fokus der aktuellen Atmosphärenforschung dar. Durch heterogene Nukleation entstehen bei Temperaturen oberhalb von -37°C primäre Eiskristalle an sogenannten eiskeimbildenden Partikeln (INP, engl, ice nucleating particles). Die räumliche Verteilung der INP und deren Quellen variieren stark. In der Atmosphäre finden sich INP nur in sehr geringer Anzahlkonzentration, oft weniger als ein Partikel pro Liter, und sie stellen nur eine kleine Untergruppe des gesamten atmosphärischen Aerosols dar. Ziel dieses Antrages ist es die Anzahlkonzentrationen von eiskeimbildenden Partikeln und deren Variabilität in der Atmosphäre zu messen. Außerdem sind Laborstudien geplant, in denen unser Verständnis über die chemischen und biologischen Eigenschaften der Partikel, die die Eisbildung initiieren, verbessert werden soll. Mit dem von unserer Arbeitsgruppe entwickelten Eiskeimzahler FINCH (Fast Ice Nucleaus CHamber) sollen die atmosphärischen Anzahlkonzentrationen von INP bei verschiedenen Gefriertemperaturen und Übersättigungen an mehreren Standorten gemessen werden. Die Kopplung von FINCH mit einem virtuellen Gegenstromimpaktor (CVI, engl, counter-flow virtual impactor, Kooperation mit RP2), die während lNUIT-1 entwickelt und getestet wurde, soll nun weiter charakterisiert und Messungen damit fortgesetzt werden. Bei dieser Methode werden die Eispartikel, die in FINCH gebildet werden, von den unterkühlten Tröpfchen und inaktivierten Partikeln separiert und mit weiteren Messmethoden untersucht. In Kooperation mit RP2 und RP8 planen wir hierbei die Charakterisierung der INP mittels Größen- und Aerosolmassenspektrometer sowie die Sammlung der INP auf Filtern oder Impaktorplatten zur anschließenden Analyse mit einem Elektronenmikroskop (ESEM, engl. DFG fomi 54.011 -04/14 page 3 of 6 Environmental Scanning Electron Microscopy). Die Feldmessdaten werden von umfangreichen Laborstudien an den Forschungseinrichtungen AIDA (RP6) und LACIS (RP7) ergänzt. Dort soll das Immersionsgefrieren von verschiedenen Testpartikeln aus biologischem Material (z.B. Zellulose), porösem Material (z.B. Zeolith) und Mineralstaub mit geringem organischem Anteil im Detail untersucht werden. Des Weiteren planen wir Labormessungen, bei denen eine verbesserte Charakterisierung der Messunsicherheiten von FINCH erarbeitet werden soll. Außerdem werden regelmäßige Tests und Kalibrierungen mit FINCH durchgeführt, für die Standardroutinen festgelegt werden sollen. Um die Rolle der INP bei der Wolken- und Niederschlagsbildung sowie bei den Wolkeneigenschaften abzuschätzen, werden die gewonnenen Messergebnisse am Ende als Eingabeparameter für erweiterte Wolkenmodelle (Kooperation mit WP-M) dienen.
Das Projekt "Contribution of ectomycorrhizal fungi to the formation and mobilization of soil organic matter (SOM)" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Helmholtz-Zentrum für Umweltforschung GmbH - UFZ, Department Umweltmikrobiologie.In forest ecosystems ectomycorrhizal fungi are responsible for the mobilization of mineral nutrients from soil organic matter (SOM) resulting in a marked increase in productivity of their symbiotic host plants. In return the fungi obtain a significant amount of photosynthetic products from these plants, allowing the formation of an extensive hyphal system. These hyphae constitute a major part of soil biomass and, ultimately, a major source for SOM formation. While plant-fungal nutrient exchange has been analyzed extensively, this proposal is focused on the fungal contribution to SOM formation and on the processes leading to the acquisition of nutrients by the fungi. These two processes will be studied separately and in a quantitative way using isotopic labeling in soil bioreactors. Analysis of the fate of 13C labeled fungal material (Laccaria bicolor) in soil bioreactors will tell how fast and to what extent the various fractions of hyphal biomass are transformed into non-living SOM. As potential molecular or structural markers for SOM formation from fungal hyphae we will analyze characteristic remnants of fungal hyphae in SOM using scanning electron microscopy, DNAfragments using a PCR approach for the fungal rRNA internal transcribed spacerregions and biochemical markers like fatty acids and ergosterol. The impact of ectomycorrhizal mycelia supported by Pinus sylvestris plantlets on 13C- and 15N-labeled SOM and on microbial biomass will be analyzed in separate soil bioreactor experiments.
Das Projekt "Das Energiewende-Szenario 2020 - Ausstieg aus der Atomenergie, Einstieg in Klimaschutz und nachhaltige Entwicklung" wird/wurde gefördert durch: Bündnis 90,Die Grünen, Bundestagsfraktion / Bündnis90 , Die Grünen im Landtag Nordrhein-Westfalen / Heinrich-Böll-Stiftung e.V.. Es wird/wurde ausgeführt durch: Öko-Institut. Institut für angewandte Ökologie e.V..
Das Projekt "Forschergruppe (FOR) 1320: Crop Sequence and Nutrient Acquisition from the Subsoil, Plant induced weathering of minerals in the subsoil - release of 'non-exchangeable' potassium from 2:1 layer minerals (TransMinK)" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Halle-Wittenberg, Institut für Agrar- und Ernährungswissenschaften, Professur für Bodenkunde und Bodenschutz.The proposal addresses the potential of subsoil to contribute to K nutrition of crops. More specifically we will address the processes controlling release of K from interlayer of 2:1 clay minerals as this is expected to be the dominant K fraction in the subsoil. While it has been shown in the past that this so called 'non-exchangeable' K can be released due to root activity, there are controversial results concerning the role of soil solution K concentration in the rhizosphere required to trigger the process. Likewise little information is available about the concentration dynamics of other cations (NH4, Ca) in the rhizosphere and their impact on K release and vermiculitization supposed to be associated with this process. Model studies with substrate from the central field trial will be conducted in compartment systems equipped with micro suction cups. The measurement of dynamic changes of soil solution composition with increasing distance from the root surface will be combined with investigations of changes in mineralogy by XRD, TEM and SEM-EDX. Changes of mineralogy as a result of plant induced K release from interlayer will also be studied on bulk soil and rhizosphere samples collected within the central field and the central microcosm experiment and with mineral bags exposed in the field during a cropping cycle. Finally, X-ray CT will be used to access changes in soil texture, i.e. clay distribution around roots and the temporal spread of roots in biopores which is a prerequisite for K uptake from such structures.
Das Projekt "Schwerpunktprogramm (SPP) 1315: Biogeochemische Grenzflächen in Böden; Biogeochemical Interfaces in Soil, Importance of soil organic carbon and mineral particle size fractions for the fate of soil supplied organic chemicals and their microbial transformations" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Johann Heinrich von Thünen-Institut Bundesforschungsinstitut für Ländliche Räume, Wald und Fischerei - Institut für Biodiversität.The biogeochemical interface (BGI) in this project is defined as the organo-mineral surface of soil particles colonized by microorganisms. In the preceding project it was demonstrated that the different soil particle size fractions were associated with specifically structured microbial communities, a characteristic amount of soil organic carbon, and a specific capacity for adsorption of the organic chemicals phenol and 2,4-dichlorophenol, respectively. While the diversity of the microbial community was responsive to fertilization-determined additional organic soil carbon in the larger particle size fractions, it was unaffected in clay. Stable isotope probing with 13C-labelled phenol and 2,4-dichlorophenol revealed that the soil organic carbon in the BGIs also affected the diversity of microorganisms involved in the degradation of these chemicals. All these results are yet only based on studying one soil with three organic carbon variants (Bad Lauchstädt) and only two organic compounds. The objective of this 2nd phase project is to apply the innovative technology developed in the 1st phase for studying the BGI processes with soil organic carbon variants from another soil (Ultuna, SPP 1315 site) and with the chiralic anilide Fungicide metalaxyl as an additional compound. This 2nd phase SPP 1315 project will also, in a collaborative effort with two other SPP 1315 partners, investigate (1) the importance of BGIs for the entantio-selective degradation of metalaxyl and (2) the role of soil microorganisms in the formation of bound residues, respectively. Furthermore, the project will utilize stable isotope probing and next-generation DNA sequencing to link the structural and functional diversity of the microbial communities responsible for metabolism of organic chemicals in the different BGIs determined by particle size fractions and soil organic carbon variants.
Das Projekt "The role of intermediate sulfur species (ISS) for isotopic fractionation processes during abiotic and chemolithoautotrophic sulfide oxidation in a natural environment" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft / Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Helmholtz-Zentrum für Umweltforschung GmbH - UFZ, Department Catchment Hydrology.Sulfur isotope fractionation (34S/32S) has been used since the late 1940s to study the chemical and biological sulfur cycle. While large isotope fractionations during bacterial sulfate reduction were used successfully to interpret, e.g., accumulation of sulfate in ancient oceans or the evolution of early life, much less is known about fractionation during sulfide oxidation. The fractionation between the two end-members sulfide and sulfate is commonly much smaller and inconsistencies exist whether substrate or product are enriched. These inconsistencies are explained by a lack of knowledge on oxidation pathways and rates as well as intermediate sulfur species, such as elemental sulfur, polysulfides, thiosulfate, sulfite, or metalloid-sulfide complexes (e.g. thioarsenates), potentially acting as 34S sinks.In the proposed project, we will develop a method for sulfur species-selective isotope analysis based on separation by preparative chromatography. Separation of Sn2- and S0 will be achieved after derivatization with methyl triflate on a C18 column, separation of the other sulfur species in an alkaline eluent on an AS16 column. Sulfur in the collected fractions will be extracted directly with activated copper chips (Sn2-, S0), or precipitated as ZnS (S2-) or BaSO4 and analyzed by routine methods as SO2. Results of this species-selective approach will be compared to those from previous techniques of end-member pool determinations and sequential precipitations.The method will be applied to sulfide oxidation profiles at neutral to alkaline hot springs at Yellowstone National Park, USA, where we detected intermediate sulfur species as important species. Determining 34S/32S only in sulfide and sulfate, our previous study has shown different fractionation patterns for two hot spring drainages with sulfide oxidation profiles that seemed similar from a geochemical perspective. The reasons for the different isotopic trends are unclear. In the present project, we will differentiate species-selective abiotic versus biotic fractionation using on-site incubation experiments with the chemolithotrophic sulfur-oxidizing bacteria Thermocrinis ruber as model organism. For selected samples, we will test whether 33S and 36S further elucidate species-selective sulfide oxidation patterns. We expect that lower source sulfide concentrations increase elemental sulfur disproportionation, thus increase redox cycling and isotope fractionation. We also expect that the larger the concentration of intermediate sulfur species, including thioarsenates, the larger the isotope fractionation. Following fractionation in species-selective pools, we will be able to clarify previously reported inconsistencies of 34S enrichment in substrate or product, elucidate sulfide oxidation pathways and rates, and reveal details about sulfur metabolism. Our new methodology and field-based data will be a basis for more consistent studies on sulfide oxidation in the future.
Das Projekt "Experimental study of the interaction of the atmosphere with polynyas/sea ice near Greenland (IKAPOS)" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Trier, Fachbereich VI Raum- und Umweltwissenschaften, Fach Umweltmeteorologie.Processes of the exchange of energy and momentum at the sea-ice/ocean/atmosphere interface are key processes for the polar climate system. Heat and moisture fluxes are strongly modulated by open water fractions associated with polynyas, having important consequences for the atmosphere, ocean processes, ice formation, brine release, gas exchange and biology. The project aims at the study of the atmospheric processes forcing and maintaining polynyas in the Greenland area. The study will focus on the interaction between the katabatic wind system and the sea-ice/ocean system in the coastal area of north and northwest Greenland, and on the atmosphere/sea-ice/ocean exchange processes over the North Water Polynya (NOW). The main tool of the experimental study will be the polar aircraft of the Alfred-Wegener Institute (AWI). The experiment will be carried out in coordination with AWI and with the Canadian ArcticNet program, and is part of the International Polar Year (IPY). The data of the experiment will be used for the validation of model simulations and parameterisations.
Das Projekt "Wind tunnel investigations on riming of ice particles: Retention of water-soluble organics and the influence of turbulence on the growth of graupels." wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Johannes Gutenberg-Universität Mainz, Institut für Physik der Atmosphäre.In recent years, gaseous and particulate organic species in the atmosphere have received increased attention. Organic compounds are found in cloud and rain water as well as in snow samples. However, the removal processes underlying their scavenging is not adequately enough understood such that these are amenable for integration in numerical models. One possibility via the ice phase would be the retention of organics during riming, i.e. the deposi-tion of super-cooled water droplets on ice particles. During this process, the organic species (or fractions of their amounts) can be transferred into and retained by the ice phase while another fraction is released back into the gas phase. These fractions will be investigated and quantified in wind tunnel experiments where freely floating ice particles and snow flakes are exposed to clouds of supercooled droplets containing organic substances of atmospheric significance. As the most abundant water-soluble organics in cloud water are carboxylic ac-ids and aldehydes the proposed experiments mainly extend over these groups. Another topic of great interest is the influence of turbulence on microphysical processes. The collisional growth of cloud droplets is significantly enhanced by turbulence; therefore, similar results are expected in the case of riming as an analogous process in the ice phase. The determination of the growth rate of ice particles by riming under turbulent conditions is necessary for the - so far not yet available - formulation of more realistic collection kernels (in comparison to the laminar case) for the modeling of cloud physics and chemistry.
Das Projekt "IPTOSS (Innovations for optimal use of organic side-streams and waste)" wird/wurde gefördert durch: Bundesamt für Umwelt. Es wird/wurde ausgeführt durch: Bundesamt für Umwelt.Biowastes and side-streams from food and beverage processing plants, such as vegetable processing and brewing are generally rich in a variety of sophisticated and valuable natural molecules i.e. cellulose, hemicellulose, lignin and pectin that have a great potential to be directly exploited in the chemical, pharmaceutical, cosmetic, feed and energy industry and/or used as biological substrates for the production of high added-value functional bioproducts. Great attention is raised toward a side-stream from the beverage industry, brewers spent grain (BSG), is here used as feedstock resource to produce fuel and valuable biomolecules. The current biowaste and side-stream valorisation and the novel biotechnological approaches for the transformation of waste into bioproducts are still relatively limited, primarily due to the lack of readily exploitable technologies. More than 33 million tonnes of BSG is generated worldwide every year. In the EU, this is close to 10 million tonnes, which equates to almost 10Prozent of the EUs annual food and drink industry waste production. Large portions of these materials are disposed by means of anaerobic digestion and composting, incineration or landfilling, the latter becoming increasingly restricted by the EU regulations in order to reduce its intrinsic environmental (i.e. greenhouse gas emission) and health hazards (Landfill Directive, 1999/31/EC, and other related EU Legislation). Eco-efficient management of organic waste is a worldwide growing issue. The groundwork for enzymatic fractionation of BSG has been already carried out in the EU project REPRO ('Reducing Food Processing Waste'). The present project is part of the European ECO-INNOVERA research program IPTOSS. The following institutions/organisations are partners in the consortium engaged in this program Aims: 1 Aim of the project The aim of the project is to develop a concept based on suitable fractionation by which the biochemical content of the material flow is better utilized for the production of new, valuable molecules. In close cooperation with the industry the sustainability of the innovation is shown and brought to the market. 2 Aims for this project of FHNW and Bionactis The aim is to develop and apply a new biocatalytic nanomaterialbased process for the value-adding depolymerization of lignocellulosic fraction of Brewers spent grain (BSG).
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