Das Projekt "Evolutionary Conflicts and their Impact on Speciation (follow-up)" wird vom Umweltbundesamt gefördert und von Eidgenössische Technische Hochschule Zürich, Institut für Integrative Biologie durchgeführt. In addition to recognizing natural selection as a universal mechanism in evolution, Darwin also saw the importance of sexual selection, yet the two have been traditionally treated largely in isolation. Here I propose to apply experimental evolution (exposing experimental populations to controlled specific selective pressures over many generations in the laboratory) to the ideally suited model system Tribolium castaneum to explore how these evolutionary forces interact and impact on the key processes underlying biodiversity. Understanding how these fundamental forces, singly and in conjunction, influence species divergence remains a major challenge in evolutionary biology. Participation of sexual selection in driving speciation is supported by substantial theoretical evidence. Theory further suggests that evolutionary conflicts (such as between the sexes or between host and parasite) might also accelerate extinction. Additional complexity is introduced by including the environmental context, linking back to natural selection. Direct experimental tests of the above concepts are essentially lacking. I will explicitly target this gap by exploiting powerful experimental evolution, incorporating the interplay between sexual selection intensity, host-parasite conflict, and adaptation to increasing temperature. Projects will assess how selection under evolutionary conflict and environmental change affects both adaptation and extinction rates, aiming to elucidate underlying mechanisms. Additionally, building on clear phenotypic divergence in key traits across experimental evolution lines, I will significantly expand on previous work by assessing patterns of divergence in gene expression, concentrating on target genes associated with reproduction, immunity and heat shock. This research will be of particular interest to scientists working in the fields of evolutionary biology and behavioural ecology, but also to ecologists, reproductive biologists, and conservation biologists. As Tribolium beetles are widespread agricultural pests, results will also be relevant to more applied researchers.