Sediment erosion and transport is critical to the ecological and commercial health of aquatic habitats from watershed to sea. There is now a consensus that microorganisms inhabiting the system mediate the erosive response of natural sediments ('ecosystem engineers') along with physicochemical properties. The biological mechanism is through secretion of a microbial organic glue (EPS: extracellular polymeric substances) that enhances binding forces between sediment grains to impact sediment stability and post-entrainment flocculation. The proposed work will elucidate the functional capability of heterotrophic bacteria, cyanobacteria and eukaryotic microalgae for mediating freshwater sediments to influence sediment erosion and transport. The potential and relevance of natural biofilms to provide this important 'ecosystem service' will be investigated for different niches in a freshwater habitat. Thereby, variations of the EPS 'quality' and 'quantity' to influence cohesion within sediments and flocs will be related to shifts in biofilm composition, sediment characteristics (e.g. organic background) and varying abiotic conditions (e.g. light, hydrodynamic regime) in the water body. Thus, the proposed interdisciplinary work will contribute to a conceptual understanding of microbial sediment engineering that represents an important ecosystem function in freshwater habitats. The research has wide implications for the water framework directive and sediment management strategies.
Cydia pomonella granulovirus (CpGV, Baculoviridae) is one of the most important agents for the control of codling moth (CM, Cydia pomonella, L.) in both biological and integrated pest management. The rapid emergence of resistance against CpGV-M, which was observed in about 40 European CM field populations from 2003 on, could be traced back to a single, dominant, sex-linked gene. Since then, resistance management has been based on mixtures of new CpGV isolates (CpGV-I12, -S), which are able to overcome this resistance. Recently, resistance even to these novel isolates was observed in CM field populations. This resistance does not follow the described dominant, sex-linked inheritance trait. At the same time, another isolate CpGV-V15 was identified showing high virulence against these resistant populations. To elucidate this novel resistance mechanism and to identify the resistance gene(s) involved, we propose a comprehensive analysis of this resistance on the cellular and genomic level of codling moth. Because of the lack of previous knowledge of the molecular mechanisms of virus resistance in insects, several different and complementary approaches will be pursued. This study will not only give an in-depth insight into the genetic possibilities for development of baculovirus resistance in CM field populations and how the virus overcomes it, but can also serve as an important model for other baculovirus-host interaction systems.
Die Nukleation von Eispartikeln spielt eine wichtige Rolle bei der Wolken- und Niederschlagsbildung, mit Konsequenten für die atmosphärische Chemie, die Wolkenphysik und das Erdklima. Für eine Quantifizierung und Vorhersage des Einflusses von Wolken in Wettervorhersage- und Klimamodellen muss die Bildung von Eispartikeln daher in einer realistischen Art und Weise beschrieben werden. Einer der wichtigen Bildungsmechanismen ist dabei die heterogene Eisnukleation im Immersionsmodus, bei dem Eis an der Oberfläche eines in einem wässrigen Tröpfchen suspendierten Eiskeims - zum Beispiel eines Mineralstaub- Partikels - gebildet wird. Wir werden im Rahmen dieses Forschungsprojekts zahlreiche Gefrierexperimente im Immersionsmodus durchführen. So werden eine Reihe verschiedener, als Aerosolpartikel in der Atmosphäre vorkommende Materialien auf ihre Eisnukleationseigenschaften hin untersucht werden. Insbesondere sollen hier die Temperatur- und Zeitabhängigkeit der von diesen Materialien ausgelösten Eisnukleation quantifiziert werden. Dabei werden wir spezielles Augenmerk auf die systematische Untersuchung der von porösen Materialien ausgelösten Eisnukleation legen. Es sollen sowohl synthetische Materialien wie beispielsweise mesoporöse Silikate untersucht werden, als auch natürlich vorkommende Materialien wie etwa mikroporöse Zeolithe.
Chlorinated ethylenes are prevalent groundwater contaminants. Numerous studies have addressed the mechanism of their reductive dehalogenation during biodegradation and reaction with zero-valent iron. However, despite insight with purified enzymes and well-characterized chemical model systems, conclusive evidence has been missing that the same mechanisms do indeed prevail in real-world transformations. While dual kinetic isotope effect measurements can provide such lines of evidence, until now this approach has not been possible for chlorinated ethylenes because an adequate method for continuous flow compound specific chlorine isotope analysis has been missing. This study attempts to close this prevalent research gap by a combination of two complementary approaches. (1) A novel analytical method to measure isotope effects for carbon and chlorine. (2) A carefully chosen set of well-defined model reactants representing distinct dehalogenation mechanisms believed to be important in real-world systems. Isotope trends observed in biotic and abiotic environmental dehalogenation will be compared to these model reactions, and the respective mechanistic hypotheses will be confirmed or discarded. With this hypothesis-driven approach it is our goal to elucidate for the first timdehalogenation reactions.
BACKGROUND: The Kingdom of Jordan belongs to the ten water scarcest countries in the world, and climate change is likely to increase the frequency of future droughts. Jordan is considered among the 10 most water impoverished countries in the world, with per capita water availability estimated at 170 m per annum, compared to an average of 1,000 m per annum in other countries. Jordan Government has taken the strategic decision to develop a conveyor system including a 325 km pipe to pump 100 million cubic meters per year of potable water from Disi-Mudawwara close to the Saudi Border in the south, to the Greater Amman area in the north. The construction of the water pipeline has started end of 2009 and shall be finished in 2013. Later on, the pipeline could serve as a major part of a national water carrier in order to convey desalinated water from the Red Sea to the economically most important central region of the country. The conveyor project will not only significantly increase water supplies to the capital, but also provide for the re-allocation of current supplies to other governorates, and for the conservation of aquifers. In the context of the Disi project that is co-funded by EIB two Environmental and Social Management Plans have been prepared: one for the private project partners and one for the Jordan Government. The latter includes the Governments obligation to re-balance water allocations to irrigation and to gradually restore the protected wetlands of Azraq (Ramsar site) east of Amman that has been depleted due to over-abstraction by re-directing discharge of highland aquifers after the Disi pipeline becomes operational. The Water Strategy recognizes that groundwater extraction for irrigation is beyond acceptable limits. Since the source is finite and priority should be given to human consumption it proposes to tackle the demand for irrigation through tariff adjustments, improved irrigation technology and disincentive to water intensive crops. The Disi aquifer is currently used for irrigation by farms producing all kinds of fruits and vegetables on a large scale and exporting most of their products to the Saudi and European markets and it is almost a third of Jordan's total consumption. The licenses for that commercial irrigation were finished by 2011/12. Whilst the licenses will be not renewed the difficulty will be the enforcement and satellite based information become an important supporting tool for monitoring. OUTLOOK: The ESA funded project Water management had the objective to support the South-North conveyor project and the activities of EIB together with the MWI in Jordan to ensure the supply of water for the increasing demand. EO Information provides a baseline for land cover and elevation and support the monitoring of further stages. usw.
In subsoils, organic matter (SOM) concentrations and microbial densities are much lower than in topsoils and most likely highly heterogeneously distributed. We therefore hypothesize, that the spatial separation between consumers (microorganisms) and their substrates (SOM) is an important limiting factor for carbon turnover in subsoils. Further, we expect microbial activity to occur mainly in few hot spots, such as the rhizosphere or flow paths where fresh substrate inputs are rapidly mineralized. In a first step, the spatial distribution of enzyme and microbial activities in top- and subsoils will be determined in order to identify hot spots and relate this to apparent 14C age, SOM composition, microbial community composition and soil properties, as determined by the other projects within the research unit. In a further step it will be determined, if microbial activity and SOM turnover is limited by substrate availability in spatially distinct soil microsites. By relating this data to root distribution and preferential flow paths we will contribute to the understanding of stabilizing and destabilizing processes of subsoil organic matter. As it is unclear, at which spatial scale these differentiating processes are effective, the analysis of spatial variability will cover the dm to the mm scale. As spatial segregation between consumers and substrates will depend on the pore and aggregate architecture of the soil, the role of the physical integrity of these structures on SOM turnover will also be investigated in laboratory experiments.
Sekundäre Partikelneubildung ist eine Hauptquelle für atmosphärische Partikel mit wichtigen Folgen für das Klima und die menschliche Gesundheit. Dieses Vorhaben untersucht die Rolle von Luft Ionen bei der sekundären Partikelneubildung in Flussreaktor- und Aerosolkammer-Experimenten unter kontrollierten Laborbedingungen. Trotz beträchtlicher Fortschritte in der Messtechnik zur Untersuchung der atmosphärischen Nukleation und des Partikelwachstums bestehen weiterhin Verständnislücken hinsichtlich der grundlegenden physikalischen und chemischen Prozesse. Insbesondere die möglichen Effekte von Ionen-Partikel-Wechselwirkungen und von Ionenchemie auf die Partikelneubildung werden kontrovers diskutiert. In Ergänzung zu bestehenden Forschungsprogrammen hinsichtlich der Rolle von Ionen im initialen Nukleationsschritt wird vorgeschlagen, Ionen-Partikel-Wechselwirkungen während des anschließenden Partikelwachstums zu untersuchen und sich dabei auf direkte Messungen des Ladungszustands, der Wachstumsraten und der chemischen Zusammensetzung von sekundärem organischem Aerosol zu konzentrieren. Hierzu werden der Ladungszustand und die Wachstumsraten von Partikelpopulationen mit einem modifizierten Mobilitätspartikelspektrometer unter wohldefinierten Randbedingungen in Laborexperimenten quantifiziert. In einem nächsten Schritt werden die neuartigen Messmöglichkeiten unseres Aerosol-Massenspektrometers CAChUP voll ausgeschöpft, um den Beitrag verschiedener organischer Vorläufergase zur chemischen Zusammensetzung von sekundärem organischen Aerosol bei variierenden Ladungszuständen zu quantifizieren. Schließlich werden die Ergebnisse dieser Experimente durch Messungen zur sekundären organischen Partikelbildung bei wohldefinierten Ionenkonzentrationen an einer Aerosolkammer überprüft. Die vorgeschlagene Forschungsagenda ist somit darauf abgestimmt, mögliche ladungs-katalysierte chemische Mechanismen bei der sekundären Aerosolbildung besser einzuordnen.
The Tropical Glaciology Group's research on Kilimanjaro started in 2002 and is in progress. Central aspects of our research plan are: 1) Development of the working hypothesis: From a synopsis of (i) proxy data indicating changes in East African climate since ca. 1850, (ii) 20th century instrumental data (temperature and precipitation), and (iii) the observations and interpretations made during two periods of fieldwork (June 2001 and July 2002) a scenario of modern glacier retreat on Kibo is reconstructed. This scenario offers the working hypothesis for our project. 2) Impact of local climate on the glaciers: This goal involves micrometeorological measurements on the glaciers, and the application of collected data to full glacier energy and mass balance models. These models quantify the impact of local climate on a glacier, based on pure physical system knowledge. Our models are validated by measured mass loss and surface temperature. 3) Latest Extent of the Kilimanjaro glaciers: Here, a satellite image was analyzed to derive the surface area and spatial distribution of glaciers on Kilimanjaro in February 2003. To validate this approach, an aerial flight was conducted in July 2005. 4) Linking local climate to large-scale circulation: As glacier behavior on Kilimanjaro, a totally free-standing mountain, is likely to reflect changes in larger-scale climate, this goal explores the large-scale climate mechanisms driving local Kilimanjaro climate. Well known large-scale forcings of east African climate are sea surface temperature variations in the Pacific and, more important, in the Indian Ocean. 5) Regional modification of large-scale circulation: The regional precipitation response in East Africa due to large-scale forcing is not adequately resolved in a global climate model as used in 4). Thus, mesoscale model experiments with the numerical atmospheric model RAMS will be conducted within this goal. They are thought to reveal the modification of atmospheric flow by the Kilimanjaro massif on a regional scale. 6) Practical aspects: Based on micro- and mesoscale results, (i) how much water is provided by glaciers, (ii) providing future projections of glacier behavior as basis for economic and societal studies (practical part), e.g., for studies on the impact of vanishing glaciers on Kibo's touristic appeal, and (iii) which impact does deforestation on the Kilimanjaro slopes have on summit climate? Referring to item 2), two new automatic weather stations have been installed in February 2005. They complete a station operated by Massachusetts University on the surface of the Northern Icefield since 2000.
The final goal of the EUROWET project is to integrate the substantial multidisciplinary European research in wetlands to help attain the sustainable management of the water cycle. This will be achieved by the translation of state-of-the art science developed at both national and European levels, into practical guidance for end-users. This will be achieved by a comprehensive review, expert assessment and a focussed dissemination strategy. There is considerable scientific knowledge and technical experience gained in diverse aspects of wetland science and management including hydrology, biogeochemistry, ecology restoration, socio-economic and policy analysis. However the results of research and management experience are still too fragmentary and not sufficiently orientated to problem-solving or simply inadequately framed to be effectively transferred to, or used by, stakeholders and policy-makers. Simultaneously the general outcome of the scientific research has been increased awareness of the significance of wetlands in delivering goods and services important for human welfare including quality of life, biodiversity conservation and maintenance or enhancement of environment quality. Despite this wetlands continue to be degraded and lost throughout Europe without adequate consideration of the wider benefits to be achieved from this management. The new Water Framework Directive (WFD) promotes a unique opportunity to redress this problem by means of the holistic, integrated approach to water management. There is currently in preparation horizontal guidance on Wetlands as part of the Common Implementation Strategy (CIS) process. There is however work still to be done on providing more specific scientific and technical guidance on the effective implementation of the Directive with respect to wetlands. This is particularly the case in relation to Integrated River Management, the CIS cluster within which wetlands are being considered in the WFD.
Durum wheat is mainly grown as a summer crop. An introduction of a winter form failed until now due to the difficulty to combine winter hardiness with required process quality. Winter hardiness is a complex trait, but in most regions the frost tolerance is decisive. Thereby a major QTL, which was found in T. monococcum, T.aestivum, H. vulgare and S.cereale on chromosome 5, seems especially important. With genotyping by sequencing it is now possible to make association mapping based on very high dense marker maps, which delivers new possibilities to detect main and epistatic effects. Furthermore, new sequencing techniques allow candidate gene based association mapping. The main aim of the project is to unravel the genetic architecture of frost tolerance and quality traits in durum. Thereby, the objectives are to (1) determine the genetic variance, heritability and correlations among frost tolerance and quality traits, (2) examine linkage disequilibrium and population structure, (3) investigate sequence polymorphism at candidate genes for frost tolerance, and (4) perform candidate gene based and genome wide association mapping.
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