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Semi-quantitative microplankton analysis at time series station Helgoland Roads, North Sea, since 2020

In order to monitor long-term variability and eventual changes in the planktonic community, a semiquantitative analysis of microplankton based on net sampling is ongoing weekly since 2020 at the Helgoland Roads station, before 9 a.m. The sampling is tightly linked to the LTER Helgoland Roads time series, thus enabling the coupling of species occurrences to the respective hydrochemical and physical conditions in the water column. These long-term data allow to quantify realized niches of frequently occurring microplankton species and to pinpoint rarely occurring species which cannot be detected in the quantitative plankton analysis.

Mesocosm experiment on the influence of heatwave on plankton

In the context of global change, marine organisms are subjected not only to gradual changes in abiotic parameters, but also to an increasing number of extreme events, such as heatwaves. However, we still know little about the influence of heatwaves on the structure of marine communities, and experimental studies are needed to test the impact of heatwaves alone, and in combination with other environmental drivers. Here, we conducted a mesocosm experiment and applied an integrated multiple driver design to assess the potential impact of heatwaves under ambient and future environmental conditions on natural coastal plankton communities. To represent future environmental conditions, temperature and pH were manipulated based on the Representative Concentration Pathway 8.5 proposed by the IPCC for 2100, and dissolved N:P ratios were increased to simulate the conditions expected in European coastal zones. Throughout the experiment, we measured abiotic conditions as well as the abundance of bacterioplankton, phytoplankton, and microzooplankton.

Health status of dab (Limanda limanda) exposed to dumped munition in German coastal waters

Conventional munition dumped into the North Sea and the Baltic Sea close to the German coastline is corroding. A major concern is that biota, including fish, are negatively affected by toxic explosives leaking into marine environments. With the present study, we investigated fish living in close proximity to munition dumping sites for contamination and for signs of health impairments. The flat fish species common dab (Limanda limanda) was used as a model, since it lives in the vicinity of dumping sites and exhibits minor migratory activity. Various health indicators (body condition factors, externally visible fish diseases, parasites or liver anomalies) were investigated.

Wasserbuch anlagenbezogen

Zu den anlagenbezogenen Wasserbucheinträgen zählen u.a. folgende wasserrechtliche Tatbestände: Benutzungen von Grundwasser und/oder Oberflächenwasser gemäß § 9 WHG i.V.m. § 5 SächsWG; Einleiten von Abwasser in Gewässer gemäß § 57 WHG (Direkteinleitung) i.V.m. § 51 SächsWG; Einleiten von Abwasser in öffentliche Abwasseranlagen gemäß § 58 WHG (Indirekteinleitung) i.V.m. § 53 SächsWG oder Einleiten von Abwasser in private Abwasseranlagen gemäß § 59 WHG; Errichtung, Betrieb, wesentliche Änderung, Unterhaltung und/oder Stilllegung von Anlagen in, an, über und unter oberirdischen Gewässern gemäß § 36 WHG i.V.m. § 26 SächsWG; Errichtung, Betrieb sowie die wesentliche Veränderung oder Beseitigung einer Abwasserbehandlungsanlage gemäß § 60 WHG i.V.m. § 55 SächsWG; Errichtung, Betrieb sowie die wesentliche Veränderung oder Beseitigung von öffentlichen Wasserversorgungsanlagen gemäß § 55 SächsWG i.V.m. § 50 Abs. 4 WHG; Nutzung von Fernwasser gemäß § 44 SächsWG i.V.m. § 50 Abs. 2 WHG; Errichtung, Betrieb und/oder wesentliche Änderung von Anlagen zum Lagern, Abfüllen oder Umschlagen wassergefährdender Stoffe gemäß § 63 WHG; Gewässerausbau sowie Errichtung von Deich- und Dammbauten gemäß § 68 WHG i.V.m. § 63 SächsWG; Herstellung, wesentlichen Änderung oder Beseitigung eines Flutungspolders gemäß § 63 SächsWG; Übertragen der Unterhaltungslast zur Gewässerunterhaltung gemäß § 40 WHG i.V.m. § 33 SächsWG, Übertragen der Pflicht zur Abwasserbeseitigung gem. § 56 WHG, Übertragen der Pflicht zur öffentlichen Wasserversorgung gemäß § 43 SächsWG; Duldungs- und Gestattungsverpflichtungen nach § 99 SächsWG (Zwangsrechte)

Wasserbuch Flächengebiet Festsetzung

Bei den Wasserbucheinträgen zur Flächengebietsfestsetzung handelt es sich u.a. um folgende wasserrechtliche Tatbestände: Wasserschutzgebiete gemäß § 51 WHG i.V.m. § 46 SächsWG; Heilquellenschutzgebiete gemäß § 53 WHG i.V.m. § 47 SächsWG; Überschwemmungsgebiete an oberirdischen Gewässern sowie vorläufig gesicherte Überschwemmungsgebiete gemäß § 76 WHG i.V.m. § 72 SächsWG; Risikogebiete gemäß § 74 WHG bzw. überschwemmungsgefährdeter Gebiete gemäß § 75 SächsWG; Hochwasserentstehungsgebiete gemäß § 78d WHG i.V.m. § 76 SächsWG; Festsetzung von Gewässerrandstreifen nach § 38 Abs. 3 WHG i.V.m. § 24 Abs. 4 SächsWG

Carbonate chemistry speciation of the 2023 KOSMOS Helgoland experiment on the effects of ocean alkalinity enhancement on pelagic foodwebs

This dataset contains carbonate chemistry speciation data of the 2023 KOSMOS mesocosm study on Helgoland, Germany. This study tested the effects of ocean alkalinity enhancement simulating lime additions on pelagic ecosystem functioning during a spring bloom. Carbonate chemistry speciation (fCO2, pHT, calcium carbonate saturation state) was generally calculated from measurements of total alkalinity (TA) and dissolved inorganic carbon (DIC) in depth-integrated water samples. There were 12 mesocosms in total and in 6 of them an alkalinity gradient of up to +1250 umol/kg was established in steps of 250 umol/kg. In the remaining 6 the same amount of alkalinity was added only to the upper portion of the mesocosms, resulting in twice the alkalinity increase there, before being mixed in after 48 hours. The two treatments simulated the immediate dilution of TA after ship deployment as well as a delayed one from a point source.

Carbonate chemistry from laboratory incubation experiments using water samples from the Elbe conducted in 2023

This dataset comprises key carbonate chemistry parameters measured and calculated in incubation experiments under different experimental conditions. pH, water temperature, and salinity were measured with a WTW multimeter (MultiLine® Multi 3630 IDS). Total alkalinity was determined by open-cell titration with an 888 Titrando (Metrohm). Saturation state of calcite and aragonite were calculated using phreeqpython, a Python wrapper of the PhreeqC engine (Vitens 2021) with pH, water temperature, total alkalinity, and major ions as major input, and phreeqc.dat as database for the thermodynamic data (Parkhurst and Appelo 2013). As the original Elbe water was supersaturated with carbon dioxide (CO2) with respect to the atmosphere, its partial pressure of CO2 (pCO2) level decreased during the incubation period with open flasks, which caused an adjustment of calcite saturation state (ΩC) for ambient air conditions. To adapt for the impact of pCO2 variations during the experiment, saturation state of calcite and aragonite was calculated assuming an equilibrium with an atmospheric pCO2 of 415 ppm (normalized ΩC and normalized aragonite sautration state ΩA). Since ion concentrations were measured for only a small number of samples, the ion concentrations of the remaining samples were reconstructed using stoichiometry based on the initial solution composition and total alkalinity. The concentrations of conservative ions (Na+, K+, Cl-, SO42-) were assumed remain constant, while ions related to carbonate precipitation (Ca2+, Mg2+) were calculated based on changes in measured alkalinity (see Figure 5 of the associated paper). Detailed analysis and calculation procedures are described in the Method section of the associated paper.

Light effects on the vertical positioning of the freshwater jellyfish (Craspedacusta sowerbii Lankester, 1880)

We experimentally manipulated the presence of light and light intensity (F = 36.7 μmol m⁻² s⁻¹; D = 0 μmol m⁻² s⁻¹; L = 4.8 μmol m⁻² s⁻¹; M = 21.4 μmol m⁻² s⁻¹) and tested their effects on the vertical positioning of the freshwater jellyfish (Craspedacusta sowerbii) medusae. For the experiments, approximately 100 C. sowerbii medusae were collected in August 2017 in two lakes (Haager Weiher and Leitner Weiher) in Bavaria, Germany. Testing was carried out at Seeon Limnological Station in close vicinity to the collection site. The experimental columns were 7.4 cm in diameter and 170 cm high and were marked with horizontal lines every 5 cm for visual position estimation. Four replicates run in parallel. One C. sowerbii medusa was used in each experimental column. Data cover three light treatments, each run twice: 1) 16:8 h full light (F)–dark (D) light intensity cycles (nF = 716, nD = 428), 2) 16:8 h full light (F)–full dark (D) light intensity cycles complemented with low (L) and medium (M) light intensities (nF = 96, nM = 96, nL = 48, nD = 288), and 3) altered light intensities in approximately 2-hour periods randomly among dark, low, medium, and full light intensities (nF = 96, nM = 76, nL = 72, nD = 336). Results show that light alone was sufficient to trigger a vertical position change of jellyfish towards the water surface, especially high light.

Physiological responses of Mytilus edulis and Magallana gigas from a three-month mesocosm experiment, Sylt, 2023

This dataset reports physiological measurements of two bivalve species, Mytilus edulis (blue mussel) and Magallana gigas (Pacific oyster), obtained during a three-month mesocosm experiment conducted in Sylt, Germany, in 2023. Physiological data were collected between 27 April 2023 and 25 June 2023.Twelve mesocosms were used to investigate the effects of temperature on individual-level physiological traits, with treatments including ambient temperature and ambient +3°C. Parameters measured include clearance rate, ingestion rate, and respiration rate. Environmental variables such as water temperature, depth, and sampling time were recorded for each measurement. Individual bivalves were labeled for tracking, and species identification followed WoRMS taxonomy. Measurements were performed using handheld multiparameter instruments and laboratory analyses. The dataset provides high-resolution, individual-based physiological responses of bivalves to moderate warming, supporting research on temperature-dependent feeding, metabolic processes, and energy flux in coastal ecosystems.

KOSMOS 2023 Helgoland mesocosm study on ocean alkalinity enhancement: sediment trap particle flux data and water column biogeochemistry

The data presented herein originates from a mesocosm study conducted as part of the BMBF CDRmare, Retake project (grant agreement no. 03F0895A), aimed at investigating the ecological ramifications of ocean alkalinity enhancement (OAE). Twelve mesocosms were deployed in Helgoland South Harbor, Germany, and systematically sampled using integrated water samplers over the period spanning from March 12th to April 20th, 2023. Six alkalinity levels under two dilution scenarios were established to differentiate between localized and uniform OAE additions. Alkalinity was increased stepwise to ΔTAmax = 1250 μmol kg-1 (250 μmol TA kg-1 increments) using sodium hydroxide (NaOH) with calcium chloride (CaCl2) to simulate cation release during calcium-based mineral dissolution, causing strong carbonate chemistry perturbations (e.g., pHT > 9.25). The dataset encompasses a spectrum of sediment trap particle flux data, water column biogeochemistry including pigment variables, inorganic nutrients, carbonate chemistry parameters. The study and data set offer insights into impacts of alkalinity enhancement on marine ecosystems and their associated biogeochemistry.

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