F1 offspring (fry) length (mm) depending on parent and offspring heatwave treatment at 30, 60 and 90 days post-hatch
F2 hatchling length (mm), clutch size (total number of eggs) and average egg diameter (mm) of the clutch depending on grandparent and parent heatwave treatment
Hatchling length (mm) upon hatching depending on parental heatwave treatment, and including average egg size of the clutch (mm) and clutch size (total number of eggs)
F1 adult clutch size (total eggs), average egg diameter (mm) plus the standard deviation, and maternal length (mm) depending on grandparent and parent heatwave treatment
Fish were brought to the laboratory at the AWI Wadden Sea Station Sylt and used as breeding adults for a multi-generation laboratory experiment investigating the effects of marine heatwaves on stickleback fitness-related traits. These F0 generation wild-caught adults (males and females) were acclimated to three heatwave scenarios (no heatwave control, single heatwave or double heatwave) for three months before breeding (starting 12 June 2022) via artificial fertilization.
Long-term survival of F1 offspring throughout the 2 year experiment (at 409, 611 and 717 days post-hatch) depending on parent and offspring heatwave treatment
We measured several fecundity traits for each clutch of fertilized eggs including clutch size (total number of eggs), average egg size (diameter (mm)), fertilization success and hatching success. The diameter (mm) of all eggs in a clutch was measured to estimate average egg size.
Activity rate (average sum of distance in cm) and total percent of arena explored by F1 offspring depending on offspring heatwave treatment
F1 offspring as adults used for breeding the F2 generation showing the number of males and females in the experiment, and the % of those that went into breeding condition depending on grandparent and parent heatwave treatment
Short-term survival of F1 offspring during the 90 day growth experiment depending on parent and offspring (beaker) heatwave treatment
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