Das Projekt "Klimaaenderungen im subalpinen-alpinen Oekoklin: Genetische Variabilitaet und adaptives Potenzial ausgewaehlter Arten" wird vom Umweltbundesamt gefördert und von Universite de Neuchatel, Institut de Botanique durchgeführt. The present project consists partly in a direct continuation of phase I of the genetic part of Ecocline, in particular by the experimental test of hypotheses formulated in its context. Moreover, it innovates by the study of two species potentially very sensitive to climate change and differing by their breeding systems. It focuses on two perspectives: a) Investigation of genetic clines along temperature gradients in order to develop tools to assess the genetic reaction of species to climate change. Clines in allele frequency along altitudinal gradients were discovered for the grass Anthoxanthum alpinum during phase I. Reciprocal transplant experiments are being performed in order to test ecotypic differentiation between low and high altitude populations of A. alpinum, together with the differential action of selection on genetic markers correlated with altitude. b) Pattern of genetic variability within and between populations as a measure of the adaptive potential of the species. Species are often considered as genetically static. Consequently, it is generally assumed that migration is the only possible alternative to extinction when new environmental conditions occur. Nevertheless, genetic variability is an important parameter for the future of a species enduring climate change. Adaptation through changes in the genetic structure of a population under new pressures of selection may allow a species to endure climate change without modifications of its range. In that respect, the variation in the number and in the size of clones by the polymorphism of AFCP in the dwarf shrub Vaccinium myrtillus (bilberry) is determined. That part will fulfill, with the use of fingerprinting techniques, the program started during the first phase of the project . That study will assess if there is a decrease in the number of clones as a function of altitude and if some clones are associated with specific environmental conditions (ecotypic differentiation). Moreover, isozyme polymorphism was investigated on one species particularly threatened by climate change, Campanula excisa, a rare monoecious endemic of central Alps present only in upper Valais in Switzerland. No correlation was found between the size of the patches, the altitude and the genetic diversity, even if genetic differences between the patches were observed. Leading Questions: Does genetic diversity change along attitudinal gradients? Are genetic markers correlated with altitude? If yes. are they submitted to selection? What will be the effect of global change on the genetic diversity of species? Is it possible to use genetic markers for monitoring the reaction of species to climate change?