This data report presents the in-situ LA-ICP-MS trace element composition of sphalerite, pyrite, and chalcopyrite from the Aouli Ag-Pb-Zn-(Cu) vein system in the Upper Moulouya district, Morocco. The dataset contributes to a study focused on characterizing the element concentrations in sulfides and elucidating the ore-forming processes at the Aouli deposit. Located within the Hercynian inlier of the Upper Moulouya, this deposit hosts one of Morocco’s largest argentiferous Pb-Zn ± F ± Ba vein-type deposits. The mineralization occurs within polyphase hydrothermal veins, oriented NE-SW to E-W and WNW-ESE. These veins occur as lodes, ore shoots, or discontinuous vein infill, with their emplacement largely controlled by structural intersections. The deposit hosts a complex mineralogical assemblage, including Ni-Co-Fe arsenides and sulfarsenides, Pb-Zn-Cu sulfides, and Ag-Sb-As sulfosalts occurring in the same vein structures, which are described for the first time. Consequently, the characterization of pyrite, chalcopyrite, and particularly sphalerite will help constrain the ore-forming process and determine the physicochemical conditions (T–fS2) of the mineralizing fluids.