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Physiological data of Pacific oyster Crassostrea (Magallana) gigas after exposure to intermittent hypoxia and the combination with (fluctuating) elevated temperature

Organisms in intertidal zones experience fluctuations in environmental stressors such as hypoxia and temperature. These stressors and their fluctuations often appear in combination. Combination of stressors can have different effects compared to single stressors. In this study, we investigate the physiological effects of intermittent hypoxia in combination with different temperature regimes on the Pacific Oyster Crassostrea (Magallana) gigas. The oysters were exposed to hypoxic cycles (12h hypoxia by emersion/12h submersion) at normal (15°C), elevated (30°C) or fluctuating (15°C submersion/30°C emersion) temperature for 10 days. After the last submersion phase, the gills and digestive gland were sampled. We measured markers for bioenergetics and redox-balance in the gills and digestive gland using colorimetric methods as well as a set of metabolites (predominantly amino acids, osmolytes, anaerobic end products and energetic metabolites) in the gills using LC-MS/MS. Oysters kept submerged for up to 10 days were used as controls.

ADCP current velocity and CTD measurements at lander stations during HE510 in the German Bight, NOAHC

Data sets contain profiles of horizontal current velocity, temperature, and salinity collected during R/V Heincke cruise HE510 in May 2018 at two locations in the German Bight: at site NOAHC and site Outer Weser (OW). Current velocities were measured by an uplooking 600 kHz RDI ADCP, which was mounted on the SEDOBS lander. ADCP transducers were located 2.2 m above the seabed. The original ping rate (mode 1) was 0.25 s-1, averaging 4 sub-pings. Data is averaged over 10 min. The cell size is 0.3 m, averaged over 1 m intervals. The vertical reference is 'meter above bed' (mab). Time is UTC. Each file contains data of one deployment (location), whereas the lander was deployed for at least 1.5 tidal cycles at each location. CTD profiles were taken during part of the lander deployment period from the anchored ship nearby and are exported on the same grid as the ADCP measurements.

ADCP current velocity and CTD measurements at lander stations during HE510 in the German Bight, OW

Data sets contain profiles of horizontal current velocity, temperature, and salinity collected during R/V Heincke cruise HE510 in May 2018 at two locations in the German Bight: at site NOAHC and site Outer Weser (OW). Current velocities were measured by an uplooking 600 kHz RDI ADCP, which was mounted on the SEDOBS lander. ADCP transducers were located 2.2 m above the seabed. The original ping rate (mode 1) was 0.25 s-1, averaging 4 sub-pings. Data is averaged over 10 min. The cell size is 0.3 m, averaged over 1 m intervals. The vertical reference is 'meter above bed' (mab). Time is UTC. Each file contains data of one deployment (location), whereas the lander was deployed for at least 1.5 tidal cycles at each location. CTD profiles were taken during part of the lander deployment period from the anchored ship nearby and are exported on the same grid as the ADCP measurements.

ADCP current velocity and CTD measurements at lander stations during HE510 in the German Bight

Data sets contain profiles of horizontal current velocity, temperature, and salinity collected during R/V Heincke cruise HE510 in May 2018 at two locations in the German Bight: at site NOAHC and site Outer Weser (OW). Current velocities were measured by an uplooking 600 kHz RDI ADCP, which was mounted on the SEDOBS lander. ADCP transducers were located 2.2 m above the seabed. The original ping rate (mode 1) was 0.25 s-1, averaging 4 sub-pings. Data is averaged over 10 min. The cell size is 0.3 m, averaged over 1 m intervals. The vertical reference is 'meter above bed' (mab). Time is UTC. Each file contains data of one deployment (location), whereas the lander was deployed for at least 1.5 tidal cycles at each location. CTD profiles were taken during part of the lander deployment period from the anchored ship nearby and are exported on the same grid as the ADCP measurements.

CTD measurements at lander stations during HE470 in the German Bight, OW

Data sets contain profiles of temperature, and salinity collected during RV HEINCKE cruise HE470 in August 2016 at two locations in the German Bight: at site NOAHC and site Outer Weser (OW). CTD profiles were taken in parallel to the lander deployment period (https://doi.org/10.1594/PANGAEA.919168) from the ship, which was anchored in a distance less than 500 m to the lander locations. CTD sensors were mounted on a microstructure probe. The casts were taken continuously and as fast as possible, resulting in typical measurement intervals of around 3 minutes between individual profiles, covering one to two tidal cycles. Small measurement uncertainties (0.002 mS cm-1 for C, 0.002 K for T) were achieved by regular calibration of the CTD sensors in the lab. Individual profiles are averaged over 10 min and 1 m in the vertical. The vertical reference is 'meter above bed' (mab). Time is UTC.

CTD measurements at lander stations during HE470 in the German Bight, NOAHC

Data sets contain profiles of temperature, and salinity collected during RV HEINCKE cruise HE470 in August 2016 at two locations in the German Bight: at site NOAHC and site Outer Weser (OW). CTD profiles were taken in parallel to the lander deployment period (https://doi.org/10.1594/PANGAEA.919168) from the ship, which was anchored in a distance less than 500 m to the lander locations. CTD sensors were mounted on a microstructure probe. The casts were taken continuously and as fast as possible, resulting in typical measurement intervals of around 3 minutes between individual profiles, covering one to two tidal cycles. Small measurement uncertainties (0.002 mS cm-1 for C, 0.002 K for T) were achieved by regular calibration of the CTD sensors in the lab. Individual profiles are averaged over 10 min and 1 m in the vertical. The vertical reference is 'meter above bed' (mab). Time is UTC.

ADCP current velocity at lander stations during HE470 in the German Bight, OUTERWESER

ADCP current velocity at lander stations during HE470 in the German Bight, NOAHC

CTD measurements at lander stations during HE470 in the German Bight

Data sets contain profiles of temperature, and salinity collected during RV HEINCKE cruise HE470 in August 2016 at two locations in the German Bight: at site NOAHC and site Outer Weser (OW). CTD profiles were taken in parallel to the lander deployment period (https://doi.org/10.1594/PANGAEA.919168) from the ship, which was anchored in a distance less than 500 m to the lander locations. CTD sensors were mounted on a microstructure probe. The casts were taken continuously and as fast as possible, resulting in typical measurement intervals of around 3 minutes between individual profiles, covering one to two tidal cycles. Small measurement uncertainties (0.002 mS cm-1 for C, 0.002 K for T) were achieved by regular calibration of the CTD sensors in the lab. Individual profiles are averaged over 10 min and 1 m in the vertical. The vertical reference is 'meter above bed' (mab). Time is UTC.

ADCP current velocity at lander stations during HE470 in the German Bight

Data sets contain horizontal current velocities, measured by an uplooking 600 kHz RDI ADCP during the FS Heincke cruise HE470 in August 2016. The device was mounted on the SEDOBS lander. Transducers were located 2.2 m above the seabed. The original ping rate (mode 1) was 0.25 s-1, averaging 4 sub-pings. Data is smoothed over 5 min, and exported in steps of 1 min. The cell size is 0.3 m. The vertical reference is 'meter above bed' (mab). Time is CEST. Each file contains data of one deployment (location), whereas the lander was deployed for at least 1.5 tidal cycles at each location.

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