Das Projekt "Aus der Atmosphäre in den Boden - wie Druckfluktuationen den Gastransport im Boden beeinflussen" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Albert-Ludwigs-Universität Freiburg, Institut für Geo- und Umweltnaturwissenschaften, Professur für Bodenökologie.Gasaustausch findet in der Atmosphäre primär durch turbulenten und laminaren Fluss statt. Im Boden dagegen spielt advektiver Gastransport eine untergeordnete Rolle, stattdessen dominiert Diffusion die Transportprozesse. Trotz der Unterschiedlichkeit und scheinbaren Unabhängigkeit dieser Prozesse wurde während Freilanduntersuchungen ein Anstieg von Gastransportraten im Boden um mehrere 10 % während Phasen starken Windes beobachtet. Dieser Anstieg ist auf wind-induzierte Druckfluktuationen zurückzuführen, die sich in das luftgefüllte Porensystem des Bodens fortpflanzen und zu einem minimal oszillierenden Luftmassenfluss führen (Pressure-pumping Effekt). Durch den oszillierenden Charakter des Luftmassenflusses ist der direkte Beitrag zum Gastransport sehr gering. Die damit einhergehende Dispersion führt jedoch zu einem Anstieg der effektiven Gastransportrate entgegen des Konzentrationsgradienten. Wird der Pressure-pumping (PP) Effekt bei der Bestimmung von Gasflüssen mit der Gradienten- und Kammermethode nicht berücksichtigt, kann dies zu großen Unsicherheiten in der Bestimmung von Bodengasflüssen führen. Insbesondere für das langfristige Monitoring von treibhausrelevanten Gasflüssen stellen diese Unsicherheiten ein zentrales Problem dar. Wir stellen vier Hypothesen auf:(H1) Der PP-Effekt ist abhängig von Bodeneigenschaften.(H2) Die Ausprägung von Luftdruckfluktuationen ist abhängig von der Rauigkeit verschiedener Landnutzungen (Wald, Grasland, landwirtschaftliche Kulturen, Stadt)(H3) Kammermessungen werden durch Luftdruckfluktuationen beeinflusst.(H4) Der Austausch und Umsatz von Methan in Böden von Mittelgebirgswäldern wird durch den PP-Effekt verstärkt. Die Hypothesen 1, 3 und 4 werden mittels Laboruntersuchungen von Proben verschiedener Böden und Bodenfeuchtebedingungen überprüft. Die Hypothese 2 wird durch Freilandmessungen an verschiedenen Standorten überprüft. Ziele des Vorhabens sind: (Z1) Modelle zu entwickeln, die die Quantifizierung des Einflusses der Bodenstruktur auf den PP-Effekt ermöglichen, (Z2) den Effekt der Oberflächenrauigkeit auf Luftdruckschwankungen zu quantifizieren, (Z3) Schwellenwerte zu definieren, die die Bestimmung von Standorten mit ausgeprägtem PP-Effekt ermöglichen, (Z4) Faktoren für die Berücksichtigung des PP-Effekts für Kammermessungen zu entwickeln, (Z5) Faktoren für die Berücksichtigung des PP-Effekts für die Gradienten Methode zu entwickeln, (Z6) den Einfluss des PP-Effekts auf die Methanaufnahme von Böden in Mittelgebirgswäldern zu bestimmen. Ein besseres Verständnis des bisher nur unzureichend untersuchten PP-Effekts wird wesentlich dazu beitragen, die Verlässlichkeit und Präzision von Messungen von Bodengasflüssen zu steigern, die die Grundlage für weitergehende Forschung darstellen.
Das Projekt "Carbon, water and nutrient dynamics in vascular plant- vs. Sphagnum-dominated bog ecosystems in southern Patagonia" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Münster, Mathematisch-Naturwissenschaftliche Fakultät, Fachbereich 14 - Geowissenschaften.In bog ecosystems, vegetation controls key processes such as the retention of carbon, water and nutrients. In northern hemispherical bogs, a shift from Sphagnum- to vascular plant-dominated vegetation is often traced back to Climate Change and increased anthropogenic nitrogen deposition and coincides with substantially reduced capacities in carbon, water and nutrient retention. In southern Patagonia, bogs dominated by Sphagnum and vascular plants coexist since millennia under similar environmental settings. Thus, South Patagonian bogs may serve as ideal examples for the long-term effect of vascular plant invasion on carbon, water and nutrient balances of bog ecosystems. The contemporary balances of carbon and water of both a bog dominated by Sphagnum and vascular plants are determined by CO2- H2O and CH4 flux measurements and an estimation of lateral water losses as well as losses via dissolved organic and inorganic carbon compounds. The high time resolution of simultaneous eddy covariance measurements of CO2 and H2O in both bog types and the strong interaction between climatic variables and the physiology of bog plants allow for direct comparisons of carbon and water fluxes during cold, warm, dry, wet, cloudy or sunny periods. By the combination with leaf-scale measurements of gas exchange and fluorescence, plant-physiological controls of photosynthesis and transpiration can be identified. Long-term peat accumulation rates will be determined by carbon density and age-depth profiles including a characterization of peat humification characteristics. A reciprocal transplantation experiment with incorporated shading, liming and labeled N addition treatments is conducted to explore driving factors affecting competition between Sphagnum and vascular plants as well as the interactions between CO2-, CH4-, and water fluxes and decisive plant functional traits affecting key processes for carbon sequestration and nutrient cycling. Decomposition rates and driving below ground processes are analyzed with a litter bag field experiment and an incubation experiment in the laboratory.
Das Projekt "Quantification of small-scale physicochemical properties of intact macropore surfaces in structured soils" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Leibniz-Zentrum für Agrarlandschaftsforschung (ZALF) e.V., Institut für Bodenlandschaftsforschung.In structured soils, the interaction of percolating water and reactive solutes with the soil matrix is mostly restricted to the surfaces of preferential flow paths. Flow paths, i.e., macropores, are formed by worm burrows, decayed root channels, cracks, and inter-aggregate spaces. While biopores are covered by earthworm casts and mucilage or by root residues, aggregates and cracks are often coated by soil organic matter (SOM), oxides, and clay minerals especially in the clay illuviation horizons of Luvisols. The SOM as well as the clay mineral composition and concentration strongly determine the wettability and sorption capacity of the coatings and thus control water and solute movement as well as the mass exchange between the preferential flow paths and the soil matrix. The objective of this proposal is the quantitative description of the small-scale distribution of physicochemical properties of intact structural surfaces and flow path surfaces and of their distribution in the soil volume. Samples of Bt horizons of Luvisols from Loess will be compared with those from glacial till. At intact structural surfaces prepared from soil clods, the spatial distribution (mm-scale) of SOM and clay mineral composition will be characterized with DRIFT (Diffuse reflectance infrared Fourier transform) spectroscopy using a self-developed mapping technique. For samples manually separated from coated surfaces and biopore walls, the contents of organic carbon (Corg) and the cation exchange capacity (CEC) will be analyzed and related to the intensities of specific signals in DRIFT spectra using Partial Least Square Regression (PLSR) analysis. The signal intensities of the DRIFT mapping spectra will be used to quantify the spatial distribution of Corg and CEC at these structural surfaces. The DRIFT mapping data will also be used for qualitatively characterizing the small scale distribution of the recalcitrance, humification, and microbial activity of the SOM from structural surfaces. The clay mineral composition of defined surface regions will be characterized by combining DRIFT spectroscopic with X-ray diffractometric analysis of manually separated samples. Subsequently, the spatial distribution of the clay mineral composition at structural surfaces will be determined from the intensities of clay mineral-specific signals in the DRIFT mapping spectra and exemplarily compared to scanning electron microscopic and infrared microscopic analysis of thin sections and thin polished micro-sections. The three-dimensional spatial distribution of the total structural surfaces in the volume of the Bt horizons will be quantified using X-ray computed tomography (CT) analysis of soil cores. The active preferential flow paths will be visualized and quantified by field tracer experiments. These CT and tracer data will be used to transfer the properties of the structural surfaces characterized by DRIFT mapping onto the active preferential flow paths in the Bt horizons.
Das Projekt "Sustainable Water Resources Management in the Yanqi Basin, Sinkiang, China" wird/wurde gefördert durch: Eidgenössische Technische Hochschule Zürich, Institut für Verkehrsplanung und Transportsysteme. Es wird/wurde ausgeführt durch: Eidgenössische Technische Hochschule Zürich, Institut für Verkehrsplanung und Transportsysteme.Irrigation in the Yanqi Basin, Sinkiang, China has led to water table rise and soil salination. A model is used to assess management options. These include more irrigation with groundwater, water saving irrigation techniques and others. The model relies on input data from remote sensing.The Yanqi Basin is located in the north-western Chinese province of Xinjiang.This agriculturally highly productive region is heavily irrigated with water drawn from the Kaidu River. The Kaidu River itself is mainly fed by snow and glacier melt from the Tian Mountain surrounding the basin. A very poor drainage system and an overexploitation of surface water have lead to a series of environmental problems: 1. Seepage water under irrigated fields has raised the groundwater table during the last years, causing strongly increased groundwater evaporation. The salt dissolved in the groundwater accumulates at the soil surface as the groundwater evaporates. This soil salinization leads to degradation of vegetation as well as to a loss of arable farmland. 2. The runoff from the Bostan Lake to the downstream Corridor is limited since large amount of water is used for irrigation in the Yanqi Basin. Nowadays, the runoff is maintained by pumping water from the lake to the river. The environmental and ecological system is facing a serious threat.In order to improve the situation in the Yanqi Basin, a jointly funded cooperation has been set up by the Institute of Environmental Engineering, Swiss Federal Institute of Technology (ETH) , China Institute of Geological and Environmental Monitoring (CIGEM) and Xinjiang Agricultural University. The situation could in principle be improved by using groundwater for irrigation, thus lowering the groundwater table and saving unproductive evaporation. However, this is associated with higher cost as groundwater has to be pumped. The major decision variable to steer the system into a desirable state is thus the ratio of irrigation water pumped from the aquifer and irrigation water drawn from the river. The basis to evaluate the ideal ratio between river and groundwater - applied to irrigation - will be a groundwater model combined with models describing the processes of the unsaturated zone. The project will focus on the following aspects of research: (...)
Das Projekt "14C content of specific organic compounds in subsoils" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität zu Köln, Institut für Geologie und Mineralogie.Organic matter (OM) composition and dynamic in subsoils is thought to be significantly different from those in surface soils. This has been suggested by increasing apparent 14C ages of bulk soil OM with depth suggesting that the amount of fresh, more easily degradable components is declining. Compositional changes have been inferred from declining ä13C values and C/N ratios indicative for stronger OM transformation. Beside these bulk OM data more specific results on OM composition and preservation mechanisms are very limited but modelling studies and results from incubation experiments suggest the presence and mineralization of younger, 'reactive carbon pool in subsoils. Less refractory OM components may be protected against degradation by interaction with soil mineral particles and within aggregates as suggested by the very limited number of more specific OM analysis e.g., identification of organic compound in soil fractions. The objective of this project is to characterize the composition, transformation, stabilization and bioavailability of OM in subsurface horizons on the molecular level: 1) major sources and compositional changes with depth will be identified by analysis of different lipid compound classes in surface and subsoil horizons, 2) the origin and stabilization of 'reactive OM will be revealed by lipid distributions and 14C values of soil fractions and of selected plant-specific lipids, and 3) organic substrates metabolized by microbial communities in subsoils are identified by distributional and 14C analysis of microbial membrane lipids. Besides detailed analyses of three soil profiles at the subsoil observatory site (Grinderwald), information on regional variability will be gained from analyses of soil profiles at sites with different parent material.
Das Projekt "The effect of elevated atmospheric CO2 concentration on gross nitrogen dynamics, plant N-uptake and microbial community dynamics in a permanent grassland" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Gießen, Institut für Pflanzenökologie (Botanik II).To predict ecosystem reactions to elevated atmospheric CO2 (eCO2) it is essential to understandthe interactions between plant carbon input, microbial community composition and activity and associated nutrient dynamics. Long-term observations (greater than 13 years) within the Giessen Free Air Carbon dioxide Enrichment (Giessen FACE) study on permanent grassland showed next to an enhanced biomass production an unexpected strong positive feedback effect on ecosystem respiration and nitrous oxide (N2O) production. The overall goal of this study is to understand the long-term effects of eCO2 and carbon input on microbial community composition and activity as well as the associated nitrogen dynamics, N2O production and plant N uptake in the Giessen FACE study on permanent grassland. A combination of 13CO2 pulse labelling with 15N tracing of 15NH4+ and 15NO3- will be carried out in situ. Different fractions of soil organic matter (recalcitrant, labile SOM) and the various mineral N pools in the soil (NH4+, NO3-, NO2-), gross N transformation rates, pool size dependent N2O and N2 emissions as well as N species dependent plant N uptake rates and the origin of the CO2 respiration will be quantified. Microbial analyses will include exploring changes in the composition of microbial communities involved in the turnover of NH4+, NO3-, N2O and N2, i.e. ammonia oxidizing, denitrifying, and microbial communities involved in dissimilatory nitrate reduction to ammonia (DNRA). Stable Isotope Probing (SIP) and mRNA based analyses will be employed to comparably evaluate the long-term effects of eCO2 on the structure and abundance of these communities, while transcripts of these genes will be used to target the fractions of the communities which actively contribute to N transformations.
Das Projekt "Forschergruppe (FOR) 1806: The Forgotten Part of Carbon Cycling: Organic Matter Storage and Turnover in Subsoils (SUBSOM), Biological Regulation of Subsoil C-cycling under Field Conditions" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Hohenheim, Institut für Bodenkunde und Standortslehre, Fachgebiet Bodenbiologie.The nature of the microbial communities inhabiting the deeper soil horizons is largely unknown. It is also not clear why subsurface microorganisms do not make faster use of organic compounds under field conditions. The answer could be provided by a reciprocal soil transfer experiment studying the response of transferred soils to fluctuations in microclimate, organic inputs, and soil biota. The subproject P9 will be responsible for the establishment of reciprocal transfer experiments offering a strong link between subgroups interested in organic matter quality, transport of organic substances, as well as functions of the soil microbial community. A single, high molecular weight substrate (13C labelled cellulose) will be applied at two different levels in the pre-experiment to understand the dose-dependent reaction of soil microorganisms in transferred surface and sub-soils. Uniformly 13C labelled beech roots - representing complex substrates - will be used for the main reciprocal soil transfer experiment. We hypothesize that transferring soil cores between subsoil and surface soil as well as addition of labelled cellulose or roots will allow us to evaluate the relative impact of surface/subsurface habitat conditions and resource availability on abundance, function, and diversity of the soil microbial community. The second objective of the subproject is to understand whether minerals buried within different soil compartments (topsoil vs. subsoil) in the field contribute to creation of hot spots of microbial abundance and activity within a period of two to five years. We hypothesize that soil microorganisms colonize organo-mineral complexes depending on their nutritional composition and substrate availability. The existence of micro-habitat specific microbial communities could be important for short term carbon storage (1 to 6 years). The third objective is to understand the biogeography and function of soil microorganisms in different subsoils. Parent material as well as mineral composition might control niche differentiation during soil development. Depending on size and interconnectedness of niches, colonization and survival of soil microbial communities might be different in soils derived from loess, sand, terra fusca, or sandstone. From the methodological point of view, our specific interest is to place community composition into context with soil microbial functions in subsoils. Our subgroup will be responsible for determining the abundance, diversity, und function of soil microorganisms (13C microbial biomass, 13C PLFA, enzyme activities, DNA extraction followed by quantitative PCR). Quantitative PCR will be used to estimate total abundances of bacteria, archaea and fungi as well as abundances of specific groups of bacteria at high taxonomic levels. We will apply taxa specific bacterial primers because classes or phyla might be differentiated into ecological categories on the basis of their life strategies.
Das Projekt "Drivers and mechanisms of 13C discrimination in Cleistogenes squarrosa (C4) - reducing uncertainties on bundle sheath leakiness" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Technische Universität München, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzung und Umwelt, Lehrstuhl für Grünlandlehre.The energetic efficiency of C4 photosynthesis is strongly affected by bundle sheath leakiness, which is commonly assessed with the 'linear version' of the Farquhar model of 13C discrimination, and leaf gas exchange and 13C composition data. But, the linear Farquhar model is a simplification of the full mechanistic theory of ? in C4 plants, potentially generating errors in the estimation of leakiness. In particular, post-photosynthetic C isotope fractionation could cause large errors, but has not been studied in any detail. The present project aims to improve the understanding of the ecological and developmental/physiological factors controlling discrimination and leakiness of the perennial grass Cleistogenes squarrosa. C. squarrosa is the most important member of the C4 community which has spread significantly in the Mongolia grasslands in the last decades. It has an unusually high and variable discrimination, which suggests very high (and potentially highly variable) leakiness. Specifically, we will conduct the first systematic study of respiratory 13C fractionation in light and dark at leaf- and stand-scale in this C4 species, and assess its effect on discrimination and estimates of leakiness. These experiments are conducted in specialized 13CO2/12CO2 gas exchange mesocosms using ecologically relevant scenarios, testing specific hypotheses on effects of environmental drivers and plant and leaf developmental stage on discrimination and leakiness.
Das Projekt "Schwerpunktprogramm (SPP) 1315: Biogeochemische Grenzflächen in Böden; Biogeochemical Interfaces in Soil, Highly-resolved imaging in artificial and natural soils to yield dynamics and structure of interfaces from oxygen, pH and water content" wird/wurde gefördert durch: Deutsche Forschungsgemeinschaft. Es wird/wurde ausgeführt durch: Universität Potsdam, Institut für Erd- und Umweltwissenschaften.In soils and sediments there is a strong coupling between local biogeochemical processes and the distribution of water, electron acceptors, acids, nutrients and pollutants. Both sides are closely related and affect each other from small scale to larger scale. Soil structures such as aggregates, roots, layers, macropores and wettability differences occurring in natural soils enhance the patchiness of these distributions. At the same time the spatial distribution and temporal dynamics of these important parameters is difficult to access. By applying non-destructive measurements it is possible to overcome these limitations. Our non-invasive fluorescence imaging technique can directly quantity distribution and changes of oxygen and pH. Similarly, the water content distribution can be visualized in situ also by optical imaging, but more precisely by neutron radiography. By applying a combined approach we will clarify the formation and architecture of interfaces induces by oxygen consumption, pH changes and water distribution. We will map and model the effects of microbial and plant root respiration for restricted oxygen supply due to locally high water saturation, in natural as well as artificial soils. Further aspects will be biologically induced pH changes, influence on fate of chemicals, and oxygen delivery from trapped gas phase.
Das Projekt "Biogeochemical Processes in Tropical Soils" wird/wurde ausgeführt durch: Universität Hohenheim, Institut für Tropische Agrarwissenschaften (Hans-Ruthenberg-Institut), Fachgebiet Pflanzenbau in den Tropen und Subtropen (490e).In recent years science has taken an increased interest in mineralization processes in tropical soils in particular under minimal tillage operations. Plant litter quality and management strongly affect mineralization-nitrification processes in soil and hence the fate of nitrogen in ecosystems and the environment. Plant secondary metabolites like lignin and polyphenols are poorly degradable and interact with proteins (protein binding capacity) and hence protect them from microbial attack. Nitrification, a microbiological process, directly and indirectly influences the efficiency of recovery of N in the vegetation as well as the loss of N (through denitrification and leaching) causing environmental pollution to water bodies and contributes to global warming (e.g. the greenhouse gas N2O is emitted as a by-product of nitrification and denitrification). Nitrifiers comprise a relatively narrow species diversity (at least as known to date) and are generally thought to be sensitive to low soil pH and stress. Despite these properties nitrification occurs in acid tropical soils with high levels of aluminium and manganese. Thus the main objective of the project will be the identification of micro-organisms and mechanisms responsible for mineralization-nitrification processes in acid tropical soils and the influence of long-term litter input of different chemical qualities and minimal tillage options. The project will include the use of stable isotopes (15N, 13C), mass spectrometry, gas chromatography (CO2, N2O), biochemical methods (PLFA) and molecular biology (16s rRNA., PCR, DGGE)
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